Qiang Wang1, Bing Zhao1, Chao Li1, Jie-Sheng Rong1, Shu-Qing Tao1, Tian-Zun Tao2. 1. 2nd Department of Orthopaedic Surgery, No. 2 Affiliated Hospital of Harbin Medical University, Heilongjiang Province 150086, China. 2. 2nd Department of Orthopaedic Surgery, No. 2 Affiliated Hospital of Harbin Medical University, Heilongjiang Province 150086, China. Electronic address: taotianzunttz@hotmail.com.
Abstract
OBJECTIVE: To explore decreased proliferation ability and differentiation potential of mesenchymal stem cells (MSCs) of osteoporosis rat. METHODS: MSCs were obtained from osteoporosis rat, and proliferation potency and impaired osteogenic differentiation potential were determined. RESULTS: The result showed a significant downregulation of MSCs pluripotency related gene (Oct 4) and osteogenic genes (BSP, OCN) expression in OVX MSCs compared with Sham MSCs (P<0.05). CONCLUSIONS: These data suggest that MSCs are aging in osteoporosis body, and autologous OVX MSCs transplantation is not appropriate to treat osteoporosis if necessary. There will be a possibility in establishing a new clinical application of MSCs autologous transplantation to treat osteoporosis, if OVX MSCs have stronger proliferation and differentiation.
OBJECTIVE: To explore decreased proliferation ability and differentiation potential of mesenchymal stem cells (MSCs) of osteoporosisrat. METHODS: MSCs were obtained from osteoporosisrat, and proliferation potency and impaired osteogenic differentiation potential were determined. RESULTS: The result showed a significant downregulation of MSCs pluripotency related gene (Oct 4) and osteogenic genes (BSP, OCN) expression in OVX MSCs compared with Sham MSCs (P<0.05). CONCLUSIONS: These data suggest that MSCs are aging in osteoporosis body, and autologous OVX MSCs transplantation is not appropriate to treat osteoporosis if necessary. There will be a possibility in establishing a new clinical application of MSCs autologous transplantation to treat osteoporosis, if OVX MSCs have stronger proliferation and differentiation.
Authors: El-Mustapha Haddouti; Thomas M Randau; Cäcilia Hilgers; Werner Masson; Robert Pflugmacher; Christof Burger; Sascha Gravius; Frank A Schildberg Journal: Int J Mol Sci Date: 2020-11-05 Impact factor: 5.923