Literature DB >> 2506153

Optimizing the immunohistochemical signal from the transferrin receptor in liver tissue.

M Lombard1, N V Naoumov, A Bomford, R Williams, M Hynes, P Dervan, J Crowe.   

Abstract

Cellular expression of the transferrin receptor is determined by the proliferative state and iron requirements of the cell. Previous immunohistochemical studies using a number of anti-(transferrin receptor) monoclonal antibodies confirmed the biochemical evidence that hepatocytes express the receptor, although the distribution shown was patchy with only a small number of cells showing positive staining. In the present study, a number of techniques have been compared to optimize detection of the immunohistochemical signal from the transferrin receptor in human liver tissue. Using an alkaline phosphatase detection system, widespread expression of this receptor with both cytoplasmic and membrane staining was found in all parenchymal and non-parenchymal cells.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2506153     DOI： 10.1007/bf01747524

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  17 in total

1.  Control of human B-lymphocyte replication. I. Characterization of novel activation states that precede the entry of G0 B cells into cycle.

Authors:  L Walker; G Guy; G Brown; M Rowe; A E Milner; J Gordon
Journal:  Immunology       Date:  1986-08       Impact factor: 7.397

2.  Liver endothelium mediates the uptake of iron-transferrin complex by hepatocytes.

Authors:  M Tavassoli; T Kishimoto; R Soda; M Kataoka; K Harjes
Journal:  Exp Cell Res       Date:  1986-08       Impact factor: 3.905

3.  Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent.

Authors:  B M Babior; R S Kipnes; J T Curnutte
Journal:  J Clin Invest       Date:  1973-03       Impact factor: 14.808

4.  A simple method of reducing the fading of immunofluorescence during microscopy.

Authors:  G D Johnson; G M Nogueira Araujo
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303

5.  Peptide mapping of the human transferrin receptor in normal and transformed cells.

Authors:  B S Stein; H H Sussman
Journal:  J Biol Chem       Date:  1983-02-25       Impact factor: 5.157

6.  A monoclonal antibody that detects expression of transferrin receptor in human erythroid precursor cells.

Authors:  D Lebman; M Trucco; L Bottero; B Lange; S Pessano; G Rovera
Journal:  Blood       Date:  1982-03       Impact factor: 22.113

7.  Demonstration of an epitope of the transferrin receptor in human cervical epithelium--a potentially useful cell marker.

Authors:  J M Lloyd; T O'Dowd; M Driver; D E Tee
Journal:  J Clin Pathol       Date:  1984-02       Impact factor: 3.411

8.  Correlation of transferrin receptor expression with histological class and outcome in non-Hodgkin lymphoma.

Authors:  J A Habeshaw; T A Lister; A G Stansfeld; M F Greaves
Journal:  Lancet       Date:  1983-03-05       Impact factor: 79.321

9.  Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin.

Authors:  R Sutherland; D Delia; C Schneider; R Newman; J Kemshead; M Greaves
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205

10.  Transferrin receptors and the uptake and release of iron by isolated hepatocytes.

Authors:  S P Young; P Aisen
Journal:  Hepatology       Date:  1981 Mar-Apr       Impact factor: 17.425
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.