Literature DB >> 25060728

Nano-scale liquid chromatography coupled to tandem mass spectrometry using the multiple reaction monitoring mode based quantitative platform for analyzing multiple enzymes associated with central metabolic pathways of Saccharomyces cerevisiae using ultra fast mass spectrometry.

Fumio Matsuda1, Tairo Ogura2, Atsumi Tomita3, Ichiro Hirano4, Hiroshi Shimizu5.   

Abstract

A widely targeted quantitative proteome analysis of Saccharomyces cerevisiae enzymes was performed employing an ultra fast mass spectrometry (UFMS). Nano-liquid chromatography-UFMS analysis of trypsin digested peptide samples derived from yeast strains successfully determined relative abundances of 303 peptides of 137 proteins.
Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Central carbon metabolism; Enzyme expression; Quantitative proteomics; Saccharomyces cerevisiae; Ultra fast mass spectrometry

Mesh：

Substances：

Year:  2014        PMID： 25060728     DOI： 10.1016/j.jbiosc.2014.06.010

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  5 in total

Review 1.  Application of targeted mass spectrometry in bottom-up proteomics for systems biology research.

Authors:  Nathan P Manes; Aleksandra Nita-Lazar
Journal:  J Proteomics       Date:  2018-02-13       Impact factor: 4.044

2.  Prediction of Hopeless Peptides Unlikely to be Selected for Targeted Proteome Analysis.

Authors:  Fumio Matsuda; Atsumi Tomita; Hiroshi Shimizu
Journal:  Mass Spectrom (Tokyo)       Date:  2017-06-02

3.  Targeted proteome analysis of single-gene deletion strains of Saccharomyces cerevisiae lacking enzymes in the central carbon metabolism.

Authors:  Fumio Matsuda; Syohei Kinoshita; Shunsuke Nishino; Atsumi Tomita; Hiroshi Shimizu
Journal:  PLoS One       Date:  2017-02-27       Impact factor: 3.240

4.  Increased carvone production in Escherichia coli by balancing limonene conversion enzyme expression via targeted quantification concatamer proteome analysis.

Authors:  Erika Yoshida; Motoki Kojima; Munenori Suzuki; Fumio Matsuda; Kazutaka Shimbo; Akiko Onuki; Yousuke Nishio; Yoshihiro Usuda; Akihiko Kondo; Jun Ishii
Journal:  Sci Rep       Date:  2021-11-11       Impact factor: 4.379

5.  Differential Proteome Analysis of Hybrid Bamboo (Bambusa pervariabilis × Dendrocalamopsis grandis) Under Fungal Stress (Arthrinium phaeospermum).

Authors:  Shujiang Li; Xinmei Fang; Shan Han; Tianhui Zhu; Hanmingyue Zhu
Journal:  Sci Rep       Date:  2019-12-10       Impact factor: 4.379
  5 in total

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