Literature DB >> 2505722

Unusually strong immunological cross-reaction between elongation factor Tu of Escherichia coli and Bacillus subtilis.

P Wenzig1, K H Schleifer.   

Abstract

Polyclonal antibodies were prepared against the purified elongation factor Tu (EF-Tu) of Escherichia coli and Bacillus subtilis. Using the methods of Western blotting and microcomplement fixation the cross-reactivities of EF-Tu of 19 different prokaryotes were determined. The immunological distance were compared with the results of 16S rRNA oligonucleotide analysis. An unexpectedly high cross-reactivity was revealed between the EF-Tu of B. subtilis and the antiserum against the EF-Tu of E. coli. A comparison of the predicted amino acid sequences from the tufgenes of E. coli and B. subtilis yielded two identical peptide fragments that are likely candidates for antibody binding sites.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2505722     DOI： 10.1007/bf00409660

Source DB:  PubMed          Journal:  Arch Microbiol        ISSN： 0302-8933            Impact factor:   2.552


  30 in total

1.  Molecular cloning and sequence determination of the tuf gene coding for the elongation factor Tu of Thermus thermophilus HB8.

Authors:  M Kushiro; M Shimizu; K Tomita
Journal:  Eur J Biochem       Date:  1987-12-30

2.  Amino acid sequence and immunological properties of chalchalaca egg white lysozyme.

Authors:  J Jollès; F Schoentgen; P Jollès; E M Prager; A C Wilson
Journal:  J Mol Evol       Date:  1976-06-23       Impact factor: 2.395

3.  Abundance and membrane association of elongation factor Tu in E. coli.

Authors:  G R Jacobson; J P Rosenbusch
Journal:  Nature       Date:  1976-05-06       Impact factor: 49.962

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Portions of the gene encoding elongation factor Tu are highly conserved in prokaryotes.

Authors:  D Filer; A V Furano
Journal:  J Biol Chem       Date:  1980-01-25       Impact factor: 5.157

6.  The complete amino-acid sequence of elongation factor Tu from Escherichia coli.

Authors:  M D Jones; T E Petersen; K M Nielsen; S Magnusson; L Sottrup-Jensen; K Gausing; B F Clark
Journal:  Eur J Biochem       Date:  1980-07

Review 7.  Antigenic structure of myoglobin: the complete immunochemical anatomy of a protein and conclusions relating to antigenic structures of proteins.

Authors:  M Z Atassi
Journal:  Immunochemistry       Date:  1975-05

8.  A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.

Authors:  M S Blake; K H Johnston; G J Russell-Jones; E C Gotschlich
Journal:  Anal Biochem       Date:  1984-01       Impact factor: 3.365

9.  The conservation of DNA sequences over very long periods of evolutionary time. Evidence against intergeneric chromosomal transfer as an explanation for the presence of Escherichia coli tuf gene sequences in taxonomically-unrelated prokaryotes.

Authors:  D Filer; R Dhar; A V Furano
Journal:  Eur J Biochem       Date:  1981-11

10.  Comparative 16S rRNA oligonucleotide analyses and murein types of round-spore-forming bacilli and non-spore-forming relatives.

Authors:  E Stackebrandt; W Ludwig; M Weizenegger; S Dorn; T J McGill; G E Fox; C R Woese; W Schubert; K H Schleifer
Journal:  J Gen Microbiol       Date:  1987-09
View more
  1 in total

1.  An epitope of elongation factor Tu is widely distributed within the bacterial and archaeal domains.

Authors:  S Weber; F Lottspeich; J Köhl
Journal:  J Bacteriol       Date:  1995-01       Impact factor: 3.490
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.