Literature DB >> 25056332

Comparison of two commercial real-time PCR assays for detection of dengue virus in patient serum samples.

J Saengsawang1, O Nathalang2, M Kamonsil3, V Watanaveeradej4.   

Abstract

This study evaluated and compared the performance of two real-time PCR assays using nested reverse transcription (RT)-PCR as the reference method. Among 117 nested RT-PCR-positive cases, the abTES DEN 5 qPCR kit detected 97.4% of dengue virus (DENV) infections, while the innuDETECT Dengue TwoStep assay did so for 44.4%. Sensitivity varied by infecting serotype and the stage of infection. The abTES kit has the potential to replace nested RT-PCR for the rapid diagnosis of DENV infection.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.

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Year:  2014        PMID： 25056332      PMCID： PMC4187784          DOI： 10.1128/JCM.01870-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus.

Authors:  J D Callahan; S J Wu; A Dion-Schultz; B E Mangold; L F Peruski; D M Watts; K R Porter; G R Murphy; W Suharyono; C C King; C G Hayes; J J Temenak
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

2.  Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction.

Authors:  R S Lanciotti; C H Calisher; D J Gubler; G J Chang; A V Vorndam
Journal:  J Clin Microbiol       Date:  1992-03       Impact factor: 5.948

Review 3.  Current advances in dengue diagnosis.

Authors:  Pei-Yun Shu; Jyh-Hsiung Huang
Journal:  Clin Diagn Lab Immunol       Date:  2004-07

Review 4.  Dengue: a continuing global threat.

Authors:  Maria G Guzman; Scott B Halstead; Harvey Artsob; Philippe Buchy; Jeremy Farrar; Duane J Gubler; Elizabeth Hunsperger; Axel Kroeger; Harold S Margolis; Eric Martínez; Michael B Nathan; Jose Luis Pelegrino; Cameron Simmons; Sutee Yoksan; Rosanna W Peeling
Journal:  Nat Rev Microbiol       Date:  2010-12       Impact factor: 60.633

5.  Cost-effective real-time reverse transcriptase PCR (RT-PCR) to screen for Dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus.

Authors:  Yee-Ling Lai; Youne-Kow Chung; Hwee-Cheng Tan; Hoon-Fang Yap; Grace Yap; Eng-Eong Ooi; Lee-Ching Ng
Journal:  J Clin Microbiol       Date:  2007-01-10       Impact factor: 5.948

6.  The measurement of observer agreement for categorical data.

Authors:  J R Landis; G G Koch
Journal:  Biometrics       Date:  1977-03       Impact factor: 2.571

7.  Validation of an internally controlled one-step real-time multiplex RT-PCR assay for the detection and quantitation of dengue virus RNA in plasma.

Authors:  Kien Duong Thi Hue; Trung Vu Tuan; Hanh Tien Nguyen Thi; Chau Tran Nguyen Bich; Huy Huynh Le Anh; Bridget A Wills; Cameron P Simmons
Journal:  J Virol Methods       Date:  2011-08-05       Impact factor: 2.014

8.  Development of group- and serotype-specific one-step SYBR green I-based real-time reverse transcription-PCR assay for dengue virus.

Authors:  Pei-Yun Shu; Shu-Fen Chang; Yu-Chung Kuo; Yi-Yun Yueh; Li-Jung Chien; Chien-Lin Sue; Ting-Hsiang Lin; Jyh-Hsiung Huang
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948
  8 in total
  5 in total

1.  Validation and Application of a Commercial Quantitative Real-Time Reverse Transcriptase-PCR Assay in Investigation of a Large Dengue Virus Outbreak in Southern Taiwan.

Authors:  Huey-Pin Tsai; You-Yuan Tsai; I-Ting Lin; Pin-Hwa Kuo; Kung-Chao Chang; Jung-Chin Chen; Wen-Chien Ko; Jen-Ren Wang
Journal:  PLoS Negl Trop Dis       Date:  2016-10-12

Review 2.  Improving Dengue Diagnostics and Management Through Innovative Technology.

Authors:  Jesus Rodriguez-Manzano; Po Ying Chia; Tsin Wen Yeo; Alison Holmes; Pantelis Georgiou; Sophie Yacoub
Journal:  Curr Infect Dis Rep       Date:  2018-06-07       Impact factor: 3.725

Review 3.  [Dengue fever : Symptoms, epidemiology, entomology, pathogen diagnosis and prevention].

Authors:  D Wiemer; H Frickmann; A Krüger
Journal:  Hautarzt       Date:  2017-12       Impact factor: 0.751

4.  Dengue and Chikungunya virus co-infection in major metropolitan cities of provinces of Punjab and Khyber Pakhtunkhwa: A multi-center study.

Authors:  Faiz Ahmed Raza; Hasnain Javed; Muhammad Mujeeb Khan; Obaid Ullah; Areeba Fatima; Muhammad Zaheer; Saima Mohsin; Shahida Hasnain; Ruqyya Khalid; Arslan Ahmed Salam
Journal:  PLoS Negl Trop Dis       Date:  2021-09-23

5.  Diagnosing the novel SARS-CoV-2 by quantitative RT-PCR: variations and opportunities.

Authors:  Horllys Gomes Barreto; Flávio Augusto de Pádua Milagres; Gessi Carvalho de Araújo; Matheus Martins Daúde; Vagner Augusto Benedito
Journal:  J Mol Med (Berl)       Date:  2020-10-17       Impact factor: 5.606
  5 in total

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