| Literature DB >> 25055788 |
Matthew A Smith1, Frances H Arnold.
Abstract
SCHEMA is a method for designing libraries of novel proteins by recombination of homologous sequences. The goal is to maximize the number of folded proteins while simultaneously generating significant sequence diversity. Here, we use the RASPP algorithm to identify optimal SCHEMA designs for shuffling contiguous elements of sequence. To exemplify the method, SCHEMA is used to recombine five fungal cellobiohydrolases (CBH1s) to produce a library of more than 390,000 novel CBH1 sequences.Mesh：
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Year: 2014 PMID： 25055788 DOI： 10.1007/978-1-4939-1053-3_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745