Angela C Weyand1, Jordan A Shavit. 1. Division of Pediatric Hematology and Oncology, Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, USA.
Abstract
PURPOSE OF REVIEW: Although the zebrafish has been established as a research tool over the past two to three decades, in hematology it has primarily been used to investigate areas distinct from coagulation. The advantages of this vertebrate model include high fecundity, rapid and external development, and conservation of virtually all clotting factors in the zebrafish genomic sequence. Here, we summarize the growing application of this technology to the study of hemostasis and thrombosis. RECENT FINDINGS: Loss of function studies have demonstrated conservation of function for a number of zebrafish coagulation factors. These include positive and negative regulators of coagulation, as well as key components of the thrombus itself, such as von Willebrand factor, fibrinogen, and thrombocytes. Such analyses have also been leveraged to aid in the understanding of human variation and disease, as well as to perform in-vivo structure/function experiments. SUMMARY: The zebrafish is an organism that lends itself to a number of unique and powerful approaches not possible in mammals. This review demonstrates that there is a high degree of genetic and functional conservation of coagulation, portending future insights into hemostasis and thrombosis through the use of this model.
PURPOSE OF REVIEW: Although the zebrafish has been established as a research tool over the past two to three decades, in hematology it has primarily been used to investigate areas distinct from coagulation. The advantages of this vertebrate model include high fecundity, rapid and external development, and conservation of virtually all clotting factors in the zebrafish genomic sequence. Here, we summarize the growing application of this technology to the study of hemostasis and thrombosis. RECENT FINDINGS: Loss of function studies have demonstrated conservation of function for a number of zebrafish coagulation factors. These include positive and negative regulators of coagulation, as well as key components of the thrombus itself, such as von Willebrand factor, fibrinogen, and thrombocytes. Such analyses have also been leveraged to aid in the understanding of human variation and disease, as well as to perform in-vivo structure/function experiments. SUMMARY: The zebrafish is an organism that lends itself to a number of unique and powerful approaches not possible in mammals. This review demonstrates that there is a high degree of genetic and functional conservation of coagulation, portending future insights into hemostasis and thrombosis through the use of this model.
Authors: Yang Liu; Colin A Kretz; Morgan L Maeder; Catherine E Richter; Philip Tsao; Andy H Vo; Michael C Huarng; Thomas Rode; Zhilian Hu; Rohit Mehra; Steven T Olson; J Keith Joung; Jordan A Shavit Journal: Blood Date: 2014-04-29 Impact factor: 22.113
Authors: J Sheehan; M Templer; M Gregory; R Hanumanthaiah; D Troyer; T Phan; B Thankavel; P Jagadeeswaran Journal: Proc Natl Acad Sci U S A Date: 2001-07-17 Impact factor: 11.205
Authors: W Y Sun; D P Witte; J L Degen; M C Colbert; M C Burkart; K Holmbäck; Q Xiao; T H Bugge; S J Degen Journal: Proc Natl Acad Sci U S A Date: 1998-06-23 Impact factor: 11.205
Authors: J Xue; Q Wu; L A Westfield; E A Tuley; D Lu; Q Zhang; K Shim; X Zheng; J E Sadler Journal: Proc Natl Acad Sci U S A Date: 1998-06-23 Impact factor: 11.205
Authors: H Jan Johnson; Manish J Gandhi; Ebrahim Shafizadeh; Nathaniel B Langer; Eric L Pierce; Barry H Paw; Diana M Gilligan; Jonathan G Drachman Journal: Exp Hematol Date: 2009-05-19 Impact factor: 3.084
Authors: Corey Cutler; Pratik Multani; David Robbins; Haesook T Kim; Thuy Le; Jonathan Hoggatt; Louis M Pelus; Caroline Desponts; Yi-Bin Chen; Betsy Rezner; Philippe Armand; John Koreth; Brett Glotzbecker; Vincent T Ho; Edwin Alyea; Marlisa Isom; Grace Kao; Myriam Armant; Leslie Silberstein; Peirong Hu; Robert J Soiffer; David T Scadden; Jerome Ritz; Wolfram Goessling; Trista E North; John Mendlein; Karen Ballen; Leonard I Zon; Joseph H Antin; Daniel D Shoemaker Journal: Blood Date: 2013-08-30 Impact factor: 22.113
Authors: Megan S Rost; Ilya Shestopalov; Yang Liu; Andy H Vo; Catherine E Richter; Sylvia M Emly; Francesca G Barrett; David L Stachura; Michael Holinstat; Leonard I Zon; Jordan A Shavit Journal: Blood Adv Date: 2018-12-11
Authors: Angela C Weyand; Steve J Grzegorski; Megan S Rost; Kari I Lavik; Allison C Ferguson; Marzia Menegatti; Catherine E Richter; Rosanna Asselta; Stefano Duga; Flora Peyvandi; Jordan A Shavit Journal: Blood Adv Date: 2019-06-11
Authors: Zhilian Hu; Yang Liu; Michael C Huarng; Marzia Menegatti; Deepak Reyon; Megan S Rost; Zachary G Norris; Catherine E Richter; Alexandra N Stapleton; Neil C Chi; Flora Peyvandi; J Keith Joung; Jordan A Shavit Journal: Blood Date: 2017-06-02 Impact factor: 22.113
Authors: Zhilian Hu; Kari I Lavik; Yang Liu; Andy H Vo; Catherine E Richter; Jorge Di Paola; Jordan A Shavit Journal: J Thromb Haemost Date: 2019-02-25 Impact factor: 5.824