BACKGROUND: The storage of red blood cells (RBC) is associated with impairment of their properties that can induce a circulatory risk to recipients. In a preceding study (2009), we reported that post-storage rejuvenation (RJ) of stored RBC (St-RBC) efficiently reduced the storage-induced RBC/endothelial cell interaction, while only partially reversing the level of intracellular Ca(2+), reactive oxygen species, and surface phosphatidylserine. In the present study, we examined the RJ effectiveness in repairing St-RBC mechanical properties. METHODS: RBC, stored in CPDA-1 without pre-storage leukoreduction, were subjected to post-storage RJ, and the deformability, osmotic fragility (OF), and mechanical fragility (MF) of the rejuvenated St-RBC (St-RBCRj) were compared to those of untreated St-RBC and of freshly-collected RBC (F-RBC). RESULTS: 5-week storage considerably increased OF and MF, and reduced the deformability of St-RBC. All alterations were only partially (40-70%) reversed by RJ, depending on the extent of the damage: the greater the damage, the lesser the relative effect of RJ. CONCLUSION: The findings of the present and preceding studies suggest that different St-RBC properties are differentially reversed by RJ, implying that some of the changes occur during storage and are irreversible.
BACKGROUND: The storage of red blood cells (RBC) is associated with impairment of their properties that can induce a circulatory risk to recipients. In a preceding study (2009), we reported that post-storage rejuvenation (RJ) of stored RBC (St-RBC) efficiently reduced the storage-induced RBC/endothelial cell interaction, while only partially reversing the level of intracellular Ca(2+), reactive oxygen species, and surface phosphatidylserine. In the present study, we examined the RJ effectiveness in repairing St-RBC mechanical properties. METHODS: RBC, stored in CPDA-1 without pre-storage leukoreduction, were subjected to post-storage RJ, and the deformability, osmotic fragility (OF), and mechanical fragility (MF) of the rejuvenated St-RBC (St-RBCRj) were compared to those of untreated St-RBC and of freshly-collected RBC (F-RBC). RESULTS: 5-week storage considerably increased OF and MF, and reduced the deformability of St-RBC. All alterations were only partially (40-70%) reversed by RJ, depending on the extent of the damage: the greater the damage, the lesser the relative effect of RJ. CONCLUSION: The findings of the present and preceding studies suggest that different St-RBC properties are differentially reversed by RJ, implying that some of the changes occur during storage and are irreversible.
Authors: Brad S Karon; Camille M van Buskirk; Elizabeth A Jaben; James D Hoyer; David D Thomas Journal: Blood Transfus Date: 2012-03-28 Impact factor: 3.443
Authors: J S Raval; J H Waters; A Seltsam; E A Scharberg; E Richter; A R Daly; M V Kameneva; M H Yazer Journal: Vox Sang Date: 2010-11 Impact factor: 2.144
Authors: Giel J C G M Bosman; Judith C A Cluitmans; Yvonne A M Groenen; Jan M Werre; Frans L A Willekens; Vĕra M J Novotný Journal: Transfusion Date: 2010-11-15 Impact factor: 3.157
Authors: Aline Roch; Nicholas J Magon; Jessica Maire; Cacang Suarna; Anita Ayer; Sophie Waldvogel; Beat A Imhof; Mark J Koury; Roland Stocker; Marc Schapira Journal: JCI Insight Date: 2019-11-01
Authors: Rebecca Radisic; Sean D Owens; Charles A Manire; Nicole Montgomery; Doug Mader; Bette Zirkelbach; Nicole I Stacy Journal: J Vet Diagn Invest Date: 2020-09-30 Impact factor: 1.279
Authors: Márcia Aires Rodrigues de Freitas; Alice Vieira da Costa; Luciana Alves de Medeiros; Mario da Silva Garrote Filho; Angélica Lemos Debs Diniz; Nilson Penha-Silva Journal: Obstet Gynecol Int Date: 2018-03-01