Literature DB >> 25053486

Notch signaling assays in Drosophila cultured cell lines.

Jinghua Li1, Benjamin E Housden, Sarah J Bray.   

Abstract

Signaling assays in Drosophila cell lines are a valuable method for investigating whether other proteins influence the function of the Notch pathway and for assessing whether specific enhancers or genes are regulated by Notch. In this chapter, we will describe two different types of assays that can be used to monitor Notch activation in Kc167 and S2 cells. One involves activating Notch in cultured cells and measuring the change in endogenous gene expression levels. The other uses luciferase reporters and measures their response to Notch, by co-transfecting with NICD.

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Year:  2014        PMID： 25053486     DOI： 10.1007/978-1-4939-1139-4_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

Review 1.  Generating and working with Drosophila cell cultures: Current challenges and opportunities.

Authors:  Arthur Luhur; Kristin M Klueg; Andrew C Zelhof
Journal:  Wiley Interdiscip Rev Dev Biol       Date:  2018-12-18       Impact factor: 5.814

Review 2.  Integration of Drosophila and Human Genetics to Understand Notch Signaling Related Diseases.

Authors:  Jose L Salazar; Shinya Yamamoto
Journal:  Adv Exp Med Biol       Date:  2018       Impact factor: 2.622

3.  HDAC1 negatively regulates selective mitotic chromatin binding of the Notch effector RBPJ in a KDM5A-dependent manner.

Authors:  Kostiantyn Dreval; Robert J Lake; Hua-Ying Fan
Journal:  Nucleic Acids Res       Date:  2019-05-21       Impact factor: 16.971

4.  TM2D genes regulate Notch signaling and neuronal function in Drosophila.

Authors:  Jose L Salazar; Sheng-An Yang; Yong Qi Lin; David Li-Kroeger; Paul C Marcogliese; Samantha L Deal; G Gregory Neely; Shinya Yamamoto
Journal:  PLoS Genet       Date:  2021-12-14       Impact factor: 5.917

5.  Ubiquitylation is required for the incorporation of the Notch receptor into intraluminal vesicles to prevent prolonged and ligand-independent activation of the pathway.

Authors:  Björn Schnute; Hideyuki Shimizu; Marvin Lyga; Martin Baron; Thomas Klein
Journal:  BMC Biol       Date:  2022-03-10       Impact factor: 7.431

6.  Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle.

Authors:  Vera Slaninova; Michaela Krafcikova; Raquel Perez-Gomez; Pavel Steffal; Lukas Trantirek; Sarah J Bray; Alena Krejci
Journal:  Open Biol       Date:  2016-02       Impact factor: 6.411
  6 in total

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