PURPOSE: The hemoglobin scavenger receptor CD163 is exclusively expressed on monocytes and tissue macrophages, also at sites of inflammation. We examined whether gallium-68 (Ga-68)-labeled anti-CD163-antibody can detect the receptor in vivo. PROCEDURES: We radiolabeled anti-CD163 antibody with Ga-68 and evaluated stability and binding specificity of the conjugate ([(68)Ga]ED2) in vitro. Furthermore, tracer biodistribution was assessed in vivo in healthy rats and rats with acute collagen-induced arthritis (CIA) by MicroPET and tissue analysis. RESULTS: Radiosynthesis of [(68)Ga]ED2 antibody yielded a tracer with high-specific activity and radiochemical purity. [(68)Ga]ED2 bound specifically to CD163 in vitro. In rats, [(68)Ga]ED2 rapidly accumulated in macrophage-rich tissues. The arthritic paws exhibited a low but significant [(68)Ga]ED2 uptake. Interestingly, the systemic distribution was also changed in the sense that a significantly higher liver uptake and lower spleen uptake of [(68)Ga]ED2 was measured in CIA rats that accordingly showed a corresponding change in level of CD163 expression. CONCLUSIONS: [(68)Ga]ED2 specifically binds CD163 in vitro and in vivo. Biodistribution studies in CIA rats suggest that this novel tool may have applications in studies of inflammatory diseases.
PURPOSE: The hemoglobin scavenger receptor CD163 is exclusively expressed on monocytes and tissue macrophages, also at sites of inflammation. We examined whether gallium-68 (Ga-68)-labeled anti-CD163-antibody can detect the receptor in vivo. PROCEDURES: We radiolabeled anti-CD163 antibody with Ga-68 and evaluated stability and binding specificity of the conjugate ([(68)Ga]ED2) in vitro. Furthermore, tracer biodistribution was assessed in vivo in healthy rats and rats with acute collagen-induced arthritis (CIA) by MicroPET and tissue analysis. RESULTS: Radiosynthesis of [(68)Ga]ED2 antibody yielded a tracer with high-specific activity and radiochemical purity. [(68)Ga]ED2 bound specifically to CD163 in vitro. In rats, [(68)Ga]ED2 rapidly accumulated in macrophage-rich tissues. The arthritic paws exhibited a low but significant [(68)Ga]ED2 uptake. Interestingly, the systemic distribution was also changed in the sense that a significantly higher liver uptake and lower spleen uptake of [(68)Ga]ED2 was measured in CIA rats that accordingly showed a corresponding change in level of CD163 expression. CONCLUSIONS: [(68)Ga]ED2 specifically binds CD163 in vitro and in vivo. Biodistribution studies in CIA rats suggest that this novel tool may have applications in studies of inflammatory diseases.
Authors: C A Ambarus; S Krausz; M van Eijk; J Hamann; T R D J Radstake; K A Reedquist; P P Tak; D L P Baeten Journal: J Immunol Methods Date: 2011-10-29 Impact factor: 2.303
Authors: Anders Etzerodt; Maciej Bogdan Maniecki; Jonas Heilskov Graversen; Holger Jon Møller; Vladimir P Torchilin; Søren Kragh Moestrup Journal: J Control Release Date: 2012-01-27 Impact factor: 9.776
Authors: Babs O Fabriek; Robin van Bruggen; Dong Mei Deng; Antoon J M Ligtenberg; Kamran Nazmi; Karin Schornagel; Rianka P M Vloet; Christine D Dijkstra; Timo K van den Berg Journal: Blood Date: 2008-10-10 Impact factor: 22.113
Authors: Machteld M J Polfliet; Babs O Fabriek; Wouter P Daniëls; Christine D Dijkstra; Timo K van den Berg Journal: Immunobiology Date: 2006-07-24 Impact factor: 3.144
Authors: Filip K Swirski; Matthias Nahrendorf; Martin Etzrodt; Moritz Wildgruber; Virna Cortez-Retamozo; Peter Panizzi; Jose-Luiz Figueiredo; Rainer H Kohler; Aleksey Chudnovskiy; Peter Waterman; Elena Aikawa; Thorsten R Mempel; Peter Libby; Ralph Weissleder; Mikael J Pittet Journal: Science Date: 2009-07-31 Impact factor: 47.728
Authors: C Sánchez-Torres; P Gómez-Puertas; M Gómez-del-Moral; F Alonso; J M Escribano; A Ezquerra; J Domínguez Journal: Arch Virol Date: 2003-09-16 Impact factor: 2.574
Authors: Kornelis S M van der Geest; Maria Sandovici; Pieter H Nienhuis; Riemer H J A Slart; Peter Heeringa; Elisabeth Brouwer; William F Jiemy Journal: Front Med (Lausanne) Date: 2022-06-06
Authors: Pia Svendsen; Jonas H Graversen; Anders Etzerodt; Henrik Hager; Rasmus Røge; Henning Grønbæk; Erik I Christensen; Holger J Møller; Hendrik Vilstrup; Søren K Moestrup Journal: Mol Ther Methods Clin Dev Date: 2016-12-24 Impact factor: 6.698
Authors: Alexander S G O'Neill; Samantha Y A Terry; Kathryn Brown; Lucy Meader; Andrew M S Wong; Jonathan D Cooper; Paul R Crocker; Wilson Wong; Gregory E D Mullen Journal: EJNMMI Res Date: 2015-11-26 Impact factor: 3.138
Authors: Martin C Boonstra; Jai Prakash; Cornelis J H Van De Velde; Wilma E Mesker; Peter J K Kuppen; Alexander L Vahrmeijer; Cornelis F M Sier Journal: Front Oncol Date: 2015-11-20 Impact factor: 6.244