| Literature DB >> 2505317 |
P Fritz1, J Hönes, D Lutz, H Multhaupt, A Mischlinski, A Dörrer, P Schwarzmann, H V Tuczek, W Müller.
Abstract
We describe three different methods of calibration of quantitative immunohistochemistry, two of them based on self-made dye-agarose or antigen-agarose substrates. Quantification of immunostained objects (immunosignals) can be done by (1) microscope photometry (plug method) or scanning-photometry; (2) and densitometry of a television transformed microscope image. Both approaches are subjected to the different methods of calibration and three examples of application.Entities:
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Year: 1989 PMID： 2505317
Source DB: PubMed Journal: Acta Histochem Suppl ISSN： 0567-7556