Mina Kamkar Asl1, Ashraf Nazariborun2, Mahmoud Hosseini3. 1. Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran. 2. Department of Physiology, School of Medicine, Zabol University of Medical Sciences, Zabol, I. R. Iran. 3. Neuroscience Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad I. R. Iran.
Abstract
OBJECTIVE: The beneficial effects of clove on toothache have been well documented. We have also previously shown the analgesic effects of clove essential oil. The present work was done to investigate the analgesic effects of the aqueous extract of clove using hot plate test. The possible role of opioid receptors in the analgesic effects of clove was also investigated using naloxone. MATERIALS AND METHODS: Ninety male mice were divided into nine groups: (1) Saline, (2-4) Aaqueous (Aq 50, Aq 100, and Aq 200) groups which were treated with 50, 100, and 200 mg/kg of aqueous extract of clove, respectively, (5-7) Ethanolic (Eth 50, Eth 100, and Eth 200) groups which were treated with 50, 100, and 200 mg/kg of ethanolic extract of clove, respectively, and (8-9) Aq 100- Naloxone and Aq 200- Naloxone which were pretreated with 4 mg/kg of naloxone before injection of 100 or 200 mg/kg of the aqueous extract. The hot plate test was performed as a base record 10 min before injection of drugs and consequently repeated every 10 minutes after the injection. RESULTS: The maximal percent effect (MPE) in the animal groups treated with 50, 100, and 200 mg/kg of aqueous extract was significantly higher than the control group. Pretreatment with naloxone reduced the analgesic effects of both 100 and 200 mg/kg of the aqueous extract. Administration of all three doses of the ethanloic extract also non-significantly increased the MPE. CONCLUSION: The results of the present study showed that aqueous extract of clove has analgesic effect in mice demonstrated by hot plate test which is reversible by naloxone. The role of opioid system in the analgesic effect of clove might be suggested. However, more investigations are needed to elucidate the exact mechanism(s).
OBJECTIVE: The beneficial effects of clove on toothache have been well documented. We have also previously shown the analgesic effects of cloveessential oil. The present work was done to investigate the analgesic effects of the aqueous extract of clove using hot plate test. The possible role of opioid receptors in the analgesic effects of clove was also investigated using naloxone. MATERIALS AND METHODS: Ninety male mice were divided into nine groups: (1) Saline, (2-4) Aaqueous (Aq 50, Aq 100, and Aq 200) groups which were treated with 50, 100, and 200 mg/kg of aqueous extract of clove, respectively, (5-7) Ethanolic (Eth 50, Eth 100, and Eth 200) groups which were treated with 50, 100, and 200 mg/kg of ethanolic extract of clove, respectively, and (8-9) Aq 100- Naloxone and Aq 200- Naloxone which were pretreated with 4 mg/kg of naloxone before injection of 100 or 200 mg/kg of the aqueous extract. The hot plate test was performed as a base record 10 min before injection of drugs and consequently repeated every 10 minutes after the injection. RESULTS: The maximal percent effect (MPE) in the animal groups treated with 50, 100, and 200 mg/kg of aqueous extract was significantly higher than the control group. Pretreatment with naloxone reduced the analgesic effects of both 100 and 200 mg/kg of the aqueous extract. Administration of all three doses of the ethanloic extract also non-significantly increased the MPE. CONCLUSION: The results of the present study showed that aqueous extract of clove has analgesic effect in mice demonstrated by hot plate test which is reversible by naloxone. The role of opioid system in the analgesic effect of clove might be suggested. However, more investigations are needed to elucidate the exact mechanism(s).
Entities:
Keywords:
Analgesia; Clove; Extract; Hot Plate; Mice; Naloxone
Authors: Myrna Déciga-Campos; Karla Lizet Beltrán-Villalobos; Hidemi Aguilar-Mariscal; María Eva González-Trujano; Guadalupe Esther Ángeles-López; Rosa Ventura-Martínez Journal: Evid Based Complement Alternat Med Date: 2021-11-11 Impact factor: 2.650
Authors: Muhammad Ikram; Amany Magdy Beshbishy; Muhammad Kifayatullah; Adedayo Olukanni; Muhammad Zahoor; Muhammad Naeem; Muhammad Amin; Masood Shah; Ahmed S Abdelaziz; Riaz Ullah; Ramzi A Mothana; Nasir A Siddiqui; Gaber El-Saber Batiha Journal: Antibiotics (Basel) Date: 2020-05-01