Tania Khan1, Merthan Unternährer2, Julia Buchholz3, Barbara Kaser-Hotz4, Bärbel Selm5, Markus Rothmaier5, Heinrich Walt1. 1. Research Division of Gynecology, Department of OB/GYN, University Hospital, Zürich CH-8091, Switzerland. 2. Research Division of Gynecology, Department of OB/GYN, University Hospital, Zürich CH-8091, Switzerland; Institute for Biomedical Engineering, ETH Zürich CH-8092, Switzerland. 3. Research Division of Gynecology, Department of OB/GYN, University Hospital, Zürich CH-8091, Switzerland; Section of Diagnostic Imaging and Radiation Oncology, VETSUISSE faculty, University of Zürich, Zürich CH-8057, Switzerland. 4. Section of Diagnostic Imaging and Radiation Oncology, VETSUISSE faculty, University of Zürich, Zürich CH-8057, Switzerland. 5. Empa - Swiss Federal Laboratories for Materials Testing and Research, Laboratory for Protection and Physiology, St. Gallen CH-9014, Switzerland.
Abstract
BACKGROUND: Medical textiles offer a unique contact opportunity that could provide value-added comfort, reliability, and safety for light or laser-based applications. We investigated a luminous textile diffuser for use in photodynamic therapy. METHODS: Textile diffusers are produced by an embroidery process. Plastic optical fibers are bent and sewn into textile to release light by macrobending. A reflective backing is incorporated to improve surface homogeneity, intensity, and safety. Clonogenic assay (MCF-7 cells) and trypan blue exclusion (NuTu19 cells) tests were performed in vitro using 0.1μg/ml m-THPC with three textile diffusers and a standard front lens diffuser. Heating effects were studied in solution and on human skin. PDT application in vivo was performed with the textile diffuser on equine sarcoids (three animals, 50mW/cm(2), 10-20J) and eight research animals. Lastly, computer simulations were performed to see how the textile diffuser might work on a curved object. RESULTS: At low fluency rate, there is a trend for the textile diffuser to have lower survival rates than the front lens diffuser for both cell lines. The textile diffuser was observed to retain more heat over a long period (>1min). All animals tolerated the treatments well and showed similar initial reactions. The simulations showed a likely focusing effect in a curved geometry. CONCLUSIONS: The initial feasibility and application using a textile-based optical diffuser has been demonstrated. Possibilities that provide additional practical advantages of the textile diffuser are discussed.
BACKGROUND: Medical textiles offer a unique contact opportunity that could provide value-added comfort, reliability, and safety for light or laser-based applications. We investigated a luminous textile diffuser for use in photodynamic therapy. METHODS: Textile diffusers are produced by an embroidery process. Plastic optical fibers are bent and sewn into textile to release light by macrobending. A reflective backing is incorporated to improve surface homogeneity, intensity, and safety. Clonogenic assay (MCF-7 cells) and trypan blue exclusion (NuTu19 cells) tests were performed in vitro using 0.1μg/ml m-THPC with three textile diffusers and a standard front lens diffuser. Heating effects were studied in solution and on human skin. PDT application in vivo was performed with the textile diffuser on equine sarcoids (three animals, 50mW/cm(2), 10-20J) and eight research animals. Lastly, computer simulations were performed to see how the textile diffuser might work on a curved object. RESULTS: At low fluency rate, there is a trend for the textile diffuser to have lower survival rates than the front lens diffuser for both cell lines. The textile diffuser was observed to retain more heat over a long period (>1min). All animals tolerated the treatments well and showed similar initial reactions. The simulations showed a likely focusing effect in a curved geometry. CONCLUSIONS: The initial feasibility and application using a textile-based optical diffuser has been demonstrated. Possibilities that provide additional practical advantages of the textile diffuser are discussed.
Authors: Sarah Chamberlain; David Bellnier; Sai Yendamuri; Joerg Lindenmann; Todd Demmy; Chukwumere Nwogu; Max Ramer; Larry Tworek; Emily Oakley; Matthew Mallory; Lindsey Carlsen; Sandra Sexton; Leslie Curtin; Gal Shafirstein Journal: Lasers Surg Med Date: 2019-10-06