Lina Xu1, Feng Luan, Huitao Liu, Yuan Gao. 1. College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005, People's Republic of China.
Abstract
BACKGROUND: Fruit and vegetables are frequently treated with fungicides to reduce possible spoilage. As a result, fungicide residues may be accumulated in derived products. This important group of chemical compounds has been heavily regulated because of their potential toxicity. Therefore, a simple and rapid method to determine fungicides is desired. RESULTS: A simple non-aqueous capillary electrophoresis (NACE) method based on dispersive liquid-liquid microextraction (DLLME) has been proposed for the determination of imazalil, prochloraz and thiabendazole fungicides in fruits and juice samples. Separation buffer consisted of a methanol-acetonitrile mixture (35:65, v/v) containing 30 mmol L⁻¹ ammonium chloride and 0.5% phosphoric acid. The optimum DLLME conditions were 80 µL trichloromethane as extraction solvent, 0.5 mL tetrahydrofuran as disperser solvent, sample solution pH at 6.0, 5% (w/v) NaCl and 10 s extraction time. Recoveries obtained for various samples ranged from 72% to 102%, with relative standard deviation lower than 6.4%. The limits of detection ranged from 0.47 to 0.72 µg kg⁻¹. CONCLUSION: The proposed method takes the advantages of DLLME and NACE. It is rapid, accurate, sensitive and reproducible for the determination of imazalil, prochloraz and thiabendazole in fruit samples.
BACKGROUND: Fruit and vegetables are frequently treated with fungicides to reduce possible spoilage. As a result, fungicide residues may be accumulated in derived products. This important group of chemical compounds has been heavily regulated because of their potential toxicity. Therefore, a simple and rapid method to determine fungicides is desired. RESULTS: A simple non-aqueous capillary electrophoresis (NACE) method based on dispersive liquid-liquid microextraction (DLLME) has been proposed for the determination of imazalil, prochloraz and thiabendazole fungicides in fruits and juice samples. Separation buffer consisted of a methanol-acetonitrile mixture (35:65, v/v) containing 30 mmol L⁻¹ ammonium chloride and 0.5% phosphoric acid. The optimum DLLME conditions were 80 µL trichloromethane as extraction solvent, 0.5 mL tetrahydrofuran as disperser solvent, sample solution pH at 6.0, 5% (w/v) NaCl and 10 s extraction time. Recoveries obtained for various samples ranged from 72% to 102%, with relative standard deviation lower than 6.4%. The limits of detection ranged from 0.47 to 0.72 µg kg⁻¹. CONCLUSION: The proposed method takes the advantages of DLLME and NACE. It is rapid, accurate, sensitive and reproducible for the determination of imazalil, prochloraz and thiabendazole in fruit samples.