Albert P Chen1, Justin Y C Lau2,3, Rohan D A Alvares4, Charles H Cunningham2,3. 1. GE Healthcare, Toronto, Canada. 2. Department of Medical Biophysics, University of Toronto, Toronto, Canada. 3. Imaging Research, Sunnybrook Research Institute, Toronto, Canada. 4. Department of Chemistry, University of Toronto, UTM, Mississauga, Canada.
Abstract
PURPOSE: Hyperpolarized [1-(13) C]lactate in solution may be a clinically relevant and safe substrate for real time MR investigations of key metabolic pathways. The potential of using hyperpolarized [1-(13) C]lactate for magnetic resonance studies of cardiac metabolism in vivo was explored. METHODS: Neat [1-(13) C]lactic acid was hyperpolarized using the dynamic nuclear polarization process. Cardiac MR spectroscopy experiments were performed in vivo using hyperpolarized [1-(13) C]lactate and [1-(13) C]pyruvate in solutions. RESULTS: A high degree of polarization was achieved for [1-(13) C]lactate in solution (16.7%). (13) C-bicarbonate was observed in rat hearts in vivo after either hyperpolarized [1-(13) C]lactate or hyperpolarized [1-(13) C]pyruvate was infused, but lower (13) C-bicarbonate to substrate ratio was observed with hyperpolarized [1-(13) C]lactate infusions. The response of (13) C-bicarbonate signal as a function of hyperpolarized [1-(13) C]lactate doses was also investigated and a saturation of (13) C-bicarbonate signal was observed at the highest dose of [1-(13) C]lactate used (0.69 mmol/kg). CONCLUSION: This study demonstrated that the use of neat [1-(13) C]lactic acid as the DNP sample is a potential alternative to [1-(13) C]pyruvic acid for cardiac hyperpolarized (13) C MR studies. Hyperpolarized [1-(13) C]lactate may enable noninvasive assessment of cardiac PDH flux in cardiac patients in the near future.
PURPOSE: Hyperpolarized [1-(13) C]lactate in solution may be a clinically relevant and safe substrate for real time MR investigations of key metabolic pathways. The potential of using hyperpolarized [1-(13) C]lactate for magnetic resonance studies of cardiac metabolism in vivo was explored. METHODS: Neat [1-(13) C]lactic acid was hyperpolarized using the dynamic nuclear polarization process. Cardiac MR spectroscopy experiments were performed in vivo using hyperpolarized [1-(13) C]lactate and [1-(13) C]pyruvate in solutions. RESULTS: A high degree of polarization was achieved for [1-(13) C]lactate in solution (16.7%). (13) C-bicarbonate was observed in rat hearts in vivo after either hyperpolarized [1-(13) C]lactate or hyperpolarized [1-(13) C]pyruvate was infused, but lower (13) C-bicarbonate to substrate ratio was observed with hyperpolarized [1-(13) C]lactate infusions. The response of (13) C-bicarbonate signal as a function of hyperpolarized [1-(13) C]lactate doses was also investigated and a saturation of (13) C-bicarbonate signal was observed at the highest dose of [1-(13) C]lactate used (0.69 mmol/kg). CONCLUSION: This study demonstrated that the use of neat [1-(13) C]lactic acid as the DNP sample is a potential alternative to [1-(13) C]pyruvic acid for cardiac hyperpolarized (13) C MR studies. Hyperpolarized [1-(13) C]lactate may enable noninvasive assessment of cardiac PDH flux in cardiac patients in the near future.
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