OBJECTIVE: α-Lipoic acid (α-LA) is a natural occurring antioxidant with beneficial effects on obesity. The aim of this study was to investigate the putative effects of α-LA on triglyceride accumulation and lipogenesis in subcutaneous adipocytes from overweight/obese subjects and to determine the potential mechanisms involved. METHODS: Fully differentiated human subcutaneous adipocytes were treated with α-LA (100 and 250 µM) during 24 h for studying triglyceride content, de novo lipogenesis, and levels of key lipogenic enzymes. The involvement of AMP-activated protein kinase (AMPK) activation was also evaluated. RESULTS: α-LA down-regulated triglyceride content by inhibiting fatty acid esterification and de novo lipogenesis. These effects were mediated by reduction in fatty acid synthase (FAS), stearoyl-coenzyme A desaturase 1, and diacylglycerol O-acyltransferase 1 protein levels. Interestingly, α-LA increased AMPK and acetyl CoA carboxylase phosphorylation, while the presence of the AMPK inhibitor Compound C reversed the inhibition observed on FAS protein levels. CONCLUSIONS: α-LA down-regulates key lipogenic enzymes, inhibiting lipogenesis and reducing triglyceride accumulation through the activation of AMPK signaling pathway in human subcutaneous adipocytes from overweight/obese subjects.
OBJECTIVE: α-Lipoic acid (α-LA) is a natural occurring antioxidant with beneficial effects on obesity. The aim of this study was to investigate the putative effects of α-LA on triglyceride accumulation and lipogenesis in subcutaneous adipocytes from overweight/obese subjects and to determine the potential mechanisms involved. METHODS: Fully differentiated human subcutaneous adipocytes were treated with α-LA (100 and 250 µM) during 24 h for studying triglyceride content, de novo lipogenesis, and levels of key lipogenic enzymes. The involvement of AMP-activated protein kinase (AMPK) activation was also evaluated. RESULTS: α-LA down-regulated triglyceride content by inhibiting fatty acid esterification and de novo lipogenesis. These effects were mediated by reduction in fatty acid synthase (FAS), stearoyl-coenzyme A desaturase 1, and diacylglycerol O-acyltransferase 1 protein levels. Interestingly, α-LA increased AMPK and acetyl CoA carboxylase phosphorylation, while the presence of the AMPK inhibitor Compound C reversed the inhibition observed on FAS protein levels. CONCLUSIONS: α-LA down-regulates key lipogenic enzymes, inhibiting lipogenesis and reducing triglyceride accumulation through the activation of AMPK signaling pathway in human subcutaneous adipocytes from overweight/obese subjects.
Authors: A E Huerta; P L Prieto-Hontoria; M Fernández-Galilea; N Sáinz; M Cuervo; J A Martínez; M J Moreno-Aliaga Journal: J Physiol Biochem Date: 2015-03-28 Impact factor: 4.158
Authors: Gerd Bobe; Alexander J Michels; Wei-Jian Zhang; Jonathan Q Purnell; Clive Woffendin; Cliff Pereira; Joseph A Vita; Nicholas O Thomas; Maret G Traber; Balz Frei; Tory M Hagen Journal: J Nutr Date: 2020-09-01 Impact factor: 4.798
Authors: L M Laiglesia; S Lorente-Cebrián; L Martínez-Fernández; N Sáinz; P L Prieto-Hontoria; M A Burrell; C M Rodríguez-Ortigosa; J A Martínez; M J Moreno-Aliaga Journal: Int J Obes (Lond) Date: 2017-09-12 Impact factor: 5.095
Authors: Esther Guiu-Jurado; Teresa Auguet; Alba Berlanga; Gemma Aragonès; Carmen Aguilar; Fàtima Sabench; Sandra Armengol; José Antonio Porras; Andreu Martí; Rosa Jorba; Mercè Hernández; Daniel del Castillo; Cristóbal Richart Journal: Int J Mol Sci Date: 2015-12-16 Impact factor: 5.923
Authors: Ana Romo-Hualde; Ana E Huerta; Carlos J González-Navarro; Omar Ramos-López; María J Moreno-Aliaga; J Alfredo Martínez Journal: Lipids Health Dis Date: 2018-05-09 Impact factor: 3.876