Monika Fraczek1, Ewa Wiland1, Malgorzata Piasecka2, Magdalena Boksa3, Dariusz Gaczarzewicz4, Anna Szumala-Kakol5, Tomasz Kolanowski1, Lothar Beutin6, Maciej Kurpisz7. 1. Institute of Human Genetics Polish Academy of Sciences, Poznan, Poland. 2. Laboratory of Histology and Developmental Biology, Pomeranian Medical University, Szczecin, Poland. 3. Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poznan, Poland. 4. Department of Animal Reproduction, Biotechnology and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, Poland. 5. Unit of Microbiology, Hospital Medical College, Poznan, Poland. 6. National Reference Laboratory for Escherichia coli, Federal Institute for Risk Assessment, Department of Biological Safety, Berlin, Germany. 7. Institute of Human Genetics Polish Academy of Sciences, Poznan, Poland. Electronic address: kurpimac@man.poznan.pl.
Abstract
OBJECTIVE: To assess the in vitro effect of three bacterial isolates (Escherichia coli, serotype O75:HNT, Staphylococcus haemolyticus, and Bacteroides ureolyticus) and/or leukocytes on sperm motility, subcellular changes in sperm plasma membranes, and sperm fertilizing potential. DESIGN: An in vitro model of semen bacterial infection. SETTING: Basic research laboratory. PATIENT(S): Healthy normozoospermic volunteers and healthy blood donors. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Sperm plasma membrane stability was evaluated with a LIVE/DEAD Sperm Viability Kit and with the merocyanine 540 (M540) test both performed using flow cytometry. An oxiSelect TBARS Assay Kit was used for quantitative measurement of malondialdehyde content. Functional ability of spermatozoa was assessed by hypo-osmotic swelling (HOS) test and sperm penetration assay (SPA). RESULT(S): The incubation of sperm with bacteria and/or leukocytes was associated with the reduction of their fertilizing potential demonstrated in both the HOS test and SPA, and this effect can be considered as a natural consequence of diminished motility and sperm membrane injury of lipid bilayers. Bacteroides ureolyticus demonstrated the most significant detrimental effect on sperm structure and function. CONCLUSION(S): Sperm motility and lipid sperm membrane status might be the earliest and the most sensitive indicators of sperm damage with negative consequences for male factor fertility, which can be attributed to both bacteria and leukocytes action.
OBJECTIVE: To assess the in vitro effect of three bacterial isolates (Escherichia coli, serotype O75:HNT, Staphylococcus haemolyticus, and Bacteroides ureolyticus) and/or leukocytes on sperm motility, subcellular changes in sperm plasma membranes, and sperm fertilizing potential. DESIGN: An in vitro model of semen bacterial infection. SETTING: Basic research laboratory. PATIENT(S): Healthy normozoospermic volunteers and healthy blood donors. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Sperm plasma membrane stability was evaluated with a LIVE/DEAD Sperm Viability Kit and with the merocyanine 540 (M540) test both performed using flow cytometry. An oxiSelect TBARS Assay Kit was used for quantitative measurement of malondialdehyde content. Functional ability of spermatozoa was assessed by hypo-osmotic swelling (HOS) test and sperm penetration assay (SPA). RESULT(S): The incubation of sperm with bacteria and/or leukocytes was associated with the reduction of their fertilizing potential demonstrated in both the HOS test and SPA, and this effect can be considered as a natural consequence of diminished motility and sperm membrane injury of lipid bilayers. Bacteroides ureolyticus demonstrated the most significant detrimental effect on sperm structure and function. CONCLUSION(S): Sperm motility and lipid sperm membrane status might be the earliest and the most sensitive indicators of sperm damage with negative consequences for male factor fertility, which can be attributed to both bacteria and leukocytes action.
Authors: Maria Torcia; Elisabetta Baldi; Sara Marchiani; Ilaria Baccani; Lara Tamburrino; Giorgio Mattiuz; Sabrina Nicolò; Chiara Bonaiuto; Carmen Panico; Linda Vignozzi; Alberto Antonelli; Gian Maria Rossolini Journal: Sci Rep Date: 2021-09-28 Impact factor: 4.379
Authors: Monika Fraczek; Angelika Lewandowska; Marta Budzinska; Marzena Kamieniczna; Lukasz Wojnar; Kamil Gill; Malgorzata Piasecka; Michal Kups; Anna Havrylyuk; Valentina Chopyak; Jozef Nakonechnyy; Andrij Nakonechnyy; Maciej Kurpisz Journal: Int J Environ Res Public Health Date: 2022-02-25 Impact factor: 3.390