Kathleen H Hong1, Hokyung Lee2, Eric J Forman3, Kathleen M Upham2, Richard T Scott3. 1. Department of Obstetrics, Gynecology and Reproductive Sciences, Rutgers-Robert Wood Johnson Medical School, New Brunswick, New Jersey; Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey. Electronic address: khong@rmanj.com. 2. Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey. 3. Department of Obstetrics, Gynecology and Reproductive Sciences, Rutgers-Robert Wood Johnson Medical School, New Brunswick, New Jersey; Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey.
Abstract
OBJECTIVE: To determine whether culture at a more physiologically cooler temperature, as suggested by limited human and animal data, would improve blastulation and pregnancy rates in human clinical IVF. DESIGN: Paired randomized controlled trial. SETTING: Academic. PATIENT(S): Infertile couples (n=52) with a female partner less than 42 years old with eight or more mature oocytes retrieved. INTERVENTION(S): Mature oocytes obtained from a single cohort of oocytes were randomly divided into two groups; one was cultured at 37°C and the other at 36°C from the time of ICSI to the time of embryo transfer or vitrification. Paired embryo transfers were accomplished by transferring one euploid embryo from each group. DNA fingerprinting was used as needed to determine the outcome for each embryo. MAIN OUTCOME MEASURE(S): Rate of development of expanded blastocysts suitable for transfer or vitrification (primary outcome), fertilization, aneuploidy, and sustained implantation. RESULT(S): A total of 805 mature oocytes were cultured; 399 at 36°C and 406 at 37°C. Paired analysis demonstrated a higher rate of usable blastocyst formation per zygote at 37°C (48.4%) vs. at 36°C (41.2%). Rates of fertilization, aneuploidy, and sustained implantation were equivalent. CONCLUSION(S): IVF culture at 36°C does not improve clinically relevant parameters of embryo development or sustained implantation rates. CLINICAL TRIAL REGISTRATION NUMBER: NCT01506089.
RCT Entities:
OBJECTIVE: To determine whether culture at a more physiologically cooler temperature, as suggested by limited human and animal data, would improve blastulation and pregnancy rates in human clinical IVF. DESIGN: Paired randomized controlled trial. SETTING: Academic. PATIENT(S): Infertile couples (n=52) with a female partner less than 42 years old with eight or more mature oocytes retrieved. INTERVENTION(S): Mature oocytes obtained from a single cohort of oocytes were randomly divided into two groups; one was cultured at 37°C and the other at 36°C from the time of ICSI to the time of embryo transfer or vitrification. Paired embryo transfers were accomplished by transferring one euploid embryo from each group. DNA fingerprinting was used as needed to determine the outcome for each embryo. MAIN OUTCOME MEASURE(S): Rate of development of expanded blastocysts suitable for transfer or vitrification (primary outcome), fertilization, aneuploidy, and sustained implantation. RESULT(S): A total of 805 mature oocytes were cultured; 399 at 36°C and 406 at 37°C. Paired analysis demonstrated a higher rate of usable blastocyst formation per zygote at 37°C (48.4%) vs. at 36°C (41.2%). Rates of fertilization, aneuploidy, and sustained implantation were equivalent. CONCLUSION(S): IVF culture at 36°C does not improve clinically relevant parameters of embryo development or sustained implantation rates. CLINICAL TRIAL REGISTRATION NUMBER: NCT01506089.
Authors: Ioannis A Sfontouris; Efstratios M Kolibianakis; George T Lainas; Christos A Venetis; George K Petsas; Basil C Tarlatzis; Tryfon G Lainas Journal: J Assist Reprod Genet Date: 2017-07-17 Impact factor: 3.412
Authors: Mara Simopoulou; Konstantinos Sfakianoudis; Anna Rapani; Polina Giannelou; George Anifandis; Stamatis Bolaris; Agni Pantou; Maria Lambropoulou; Athanasios Pappas; Efthimios Deligeoroglou; Konstantinos Pantos; Michael Koutsilieris Journal: In Vivo Date: 2018 May-Jun Impact factor: 2.155