Hao-Yuan Cheng1, Fu-Hsiang Ko1, Lee-Jene Lai2. 1. Department of Materials Science and Engineering, National Chiao Tung University, Taiwan, ROC. 2. National Synchrotron Radiation Research Center, Taiwan, ROC.
Abstract
BACKGROUND: Synchrotron radiation infrared (SR-IR) microspectroscopy and SR-IR spectroscopic imaging are extremely valuable techniques for determining the molecular composition of biological and biomedical samples. In this work, SR-IR is applied in the study of the lung cancer cells in different cell cycles. METHODS: We use a novel synchrotron based radiation infrared system combined synchronized model human lung carcinoma to reveal its unique character pattern. RESULTS: After using SR-IR microspectroscopy, we discovered that the ratio of protein to lipid in G1 and G2 states is around 4.0 and 6.1, respectively. Moreover, for the DNA at the wavenumber position of 1225 cm(-1) , the intensity ratio of G2 state to G1 state is approximately 1.6. These data indicate that the cell in G1 state has more lipid composition to prepare for the DNA synthesis, but the cell in G2 state has more protein composition to prepare for the mitosis. The cell has larger DNA concentration in G2 state, which can be explained for the DNA synthesis. CONCLUSION: Through our research, we demonstrate that different growth state of cancer cell presenting unique functional groups concentration profiles and distribution via using SR-IR microspectrometry. These applications will provide another ways to improve modern cancer screening in the future.
BACKGROUND: Synchrotron radiation infrared (SR-IR) microspectroscopy and SR-IR spectroscopic imaging are extremely valuable techniques for determining the molecular composition of biological and biomedical samples. In this work, SR-IR is applied in the study of the lung cancer cells in different cell cycles. METHODS: We use a novel synchrotron based radiation infrared system combined synchronized model humanlung carcinoma to reveal its unique character pattern. RESULTS: After using SR-IR microspectroscopy, we discovered that the ratio of protein to lipid in G1 and G2 states is around 4.0 and 6.1, respectively. Moreover, for the DNA at the wavenumber position of 1225 cm(-1) , the intensity ratio of G2 state to G1 state is approximately 1.6. These data indicate that the cell in G1 state has more lipid composition to prepare for the DNA synthesis, but the cell in G2 state has more protein composition to prepare for the mitosis. The cell has larger DNA concentration in G2 state, which can be explained for the DNA synthesis. CONCLUSION: Through our research, we demonstrate that different growth state of cancer cell presenting unique functional groups concentration profiles and distribution via using SR-IR microspectrometry. These applications will provide another ways to improve modern cancer screening in the future.
Authors: Ehsan Gazi; Matthew Baker; John Dwyer; Nicholas P Lockyer; Peter Gardner; Jonathan H Shanks; Roy S Reeve; Claire A Hart; Noel W Clarke; Michael D Brown Journal: Eur Urol Date: 2006-03-31 Impact factor: 20.096
Authors: Donald C Malins; Paul M Johnson; Edward A Barker; Nayak L Polissar; Thomas M Wheeler; Katie M Anderson Journal: Proc Natl Acad Sci U S A Date: 2003-04-17 Impact factor: 11.205
Authors: S Mordechai; R K Sahu; Z Hammody; S Mark; K Kantarovich; H Guterman; A Podshyvalov; J Goldstein; S Argov Journal: J Microsc Date: 2004-07 Impact factor: 1.758