Automated microscopic image analysis for leukocytes identification: a survey.

Automatic quantification and classification of leukocytes in microscopic images are of paramount importance in the perspective of disease identification, its progress and drugs development. Extracting numerical values of leukocytes from microscopic images of blood or tissue sections represents a tricky challenge. Research efforts in quantification of these cells include normalization of images, segmentation of its nuclei and cytoplasm followed by their classification. However, there are several related problems viz., coarse background, overlapped nuclei, conversion of 3-D nuclei into 2-D nuclei etc. In this review, we have categorized, evaluated, and discussed recently developed methods for leukocyte identification. After reviewing these methods and finding their constraints, a future research perspective has been presented. Further, the challenges faced by the pathologists with respect to these problems are also discussed.