Literature DB >> 2504146

Purification of a unique glycoprotein that enhances phenol oxidase activity in scorpion (Heterometrus bengalensis) haemolymph.

T K Datta1, P S Basu, P K Datta, A Banerjee.   

Abstract

A monomeric glycoprotein (SGP) of Mr 32,000 was isolated to purity from scorpion (Heterometrus bengalensis) haemolymph by (NH4)2SO4 fractionation, chromatofocusing and h.p.l.c. The homogeneity of SGP is confirmed by polyacrylamide-gel electrophoresis. SGP is soluble in 100%-satd. (NH4)2SO4 solution. Needle-shaped crystals of SGP were obtained in an aqueous environment. The glycan part of the molecule contains arabinose, which does not commonly occur in animal glycoproteins. Amino acid analysis demonstrated a preponderance of glycine, tyrosine and glutamic acid. SGP enhances phenol oxidase (EC 1.14.18.1) activity.

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Year:  1989        PMID： 2504146      PMCID： PMC1138699          DOI： 10.1042/bj2600525

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  A spectrophotometric determination of trypsin and chymotrypsin.

Authors:  G W SCHWERT; Y TAKENAKA
Journal:  Biochim Biophys Acta       Date:  1955-04

3.  Alcian blue staining of glycoproteins in acrylamide disc electrophoresis.

Authors:  A H Wardi; G A Michos
Journal:  Anal Biochem       Date:  1972-10       Impact factor: 3.365

4.  Glycoprotein staining following electrophoresis on acrylamide gels.

Authors:  R M Zacharius; T E Zell; J H Morrison; J J Woodlock
Journal:  Anal Biochem       Date:  1969-07       Impact factor: 3.365

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Endogenous protease inhibitors prevent undesired activation of prophenolase in insect hemolymph.

Authors:  M Sugumaran; S J Saul; N Ramesh
Journal:  Biochem Biophys Res Commun       Date:  1985-11-15       Impact factor: 3.575

7.  Chromatofocusing separation of functional units of scorpion hemocyanin and their Ca2+ binding characteristics.

Authors:  P K Datta; P S Basu; T K Datta
Journal:  Indian J Biochem Biophys       Date:  1985-12       Impact factor: 1.918

8.  Purification and characterization of a high-Mr proteinase inhibitor of pro-phenol oxidase activation from crayfish plasma.

Authors:  H G Hergenhahn; A Aspan; K Söderhäll
Journal:  Biochem J       Date:  1987-11-15       Impact factor: 3.857

Review 9.  Hemocyanin--a current perspective.

Authors:  H D Ellerton; N F Ellerton; H A Robinson
Journal:  Prog Biophys Mol Biol       Date:  1983       Impact factor: 3.667

10.  Purification and partial characterization of an erythroagglutinin from the hemolymph of scorpion, Heterometrus bengalensis.

Authors:  P S Basu; P K Datta; O P Agarwal; M K Ray; T K Datta
Journal:  Biochimie       Date:  1984-06       Impact factor: 4.079
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