H Xiong1, L Wei1, B Peng1. 1. State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Abstract
AIM: To investigate IL-17 expression in human pulpitis and to study the effects of IL-17 on the secretion of the chemokines IL-6 and IL-8 and the related signalling pathways. METHODOLOGY: Samples of human dental pulp tissue were obtained from healthy controls and patients with pulpitis. Cytokine IL-17 messenger RNA (mRNA) expression in the pulp tissue was detected by real-time polymerase chain reaction. In addition, human dental pulp fibroblasts (HDPFs) were stimulated with IL-17. Production of IL-6 and IL-8 was determined by reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay. Aspects of the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) signalling pathways were examined by Western blot analysis. RESULT: Increased levels of IL-17 mRNA were found in inflamed dental pulp tissue (pulpitis). Stimulation of dental pulp tissue with IL-17 induced the production of IL-6 and IL-8 in a dose-dependent manner. In addition, IL-17 stimulation resulted in rapid activation of nuclear factor-kappaB (NF-κB), phosphorylation of p38 MAPK, extracellular signal-regulated kinase (ERK) and Jun N-terminal kinase (JNK) in HDPFs. CONCLUSION: IL-17 may participate in pulp tissue inflammation through chemokine production and NF-κB and MAPKs signalling pathways.
AIM: To investigate IL-17 expression in humanpulpitis and to study the effects of IL-17 on the secretion of the chemokines IL-6 and IL-8 and the related signalling pathways. METHODOLOGY: Samples of human dental pulp tissue were obtained from healthy controls and patients with pulpitis. Cytokine IL-17 messenger RNA (mRNA) expression in the pulp tissue was detected by real-time polymerase chain reaction. In addition, human dental pulp fibroblasts (HDPFs) were stimulated with IL-17. Production of IL-6 and IL-8 was determined by reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay. Aspects of the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) signalling pathways were examined by Western blot analysis. RESULT: Increased levels of IL-17 mRNA were found in inflamed dental pulp tissue (pulpitis). Stimulation of dental pulp tissue with IL-17 induced the production of IL-6 and IL-8 in a dose-dependent manner. In addition, IL-17 stimulation resulted in rapid activation of nuclear factor-kappaB (NF-κB), phosphorylation of p38 MAPK, extracellular signal-regulated kinase (ERK) and Jun N-terminal kinase (JNK) in HDPFs. CONCLUSION:IL-17 may participate in pulp tissue inflammation through chemokine production and NF-κB and MAPKs signalling pathways.
Authors: Qiannan Sun; Qian Wang; Ning Feng; Yan Meng; Bin Li; Demei Luo; Xiaoqian Shang; Jie Lv; Ahmad Maqsuod Monsaf; Changmin Wang; Xiumin Ma Journal: Ann Transl Med Date: 2019-08
Authors: Shelly Arora; Paul R Cooper; Lara T Friedlander; Benedict Seo; Shakila B Rizwan; Alison M Rich; Haizal Mohd Hussaini Journal: Int J Mol Sci Date: 2022-08-20 Impact factor: 6.208