PURPOSE: Human papillomavirus (HPV) 16 infection is associated with oropharyngeal carcinogenesis and is likely the cause of the reported increase in disease incidence. We evaluated the prevalence of HPV infection and the reliability of different diagnostic tools using primary tumor samples from a cohort of 50 patients. METHODS AND MATERIALS: Formalin-fixed paraffin-embedded (FFPE) tumor samples were collected from all 50 consecutive primary oropharyngeal SCC patients who were enrolled in the study; fresh tumor samples were available in 22 cases. NucliSENS EasyQ HPVv1 was used for RNA, and Digene Hybrid Capture-2(HC2) was used for DNA detection. p16 Expression was evaluated by immunohistochemistry in FPPE specimens. RESULTS: Based on the DNA detection assay on FFPE samples, the frequency of high-risk HPV infection was 32%. The agreement rate between HPV RNA and HPV DNA detection in fresh samples was 100%. The agreement rate between p16 immunohistochemistry (IHC) and the detection of HPV DNA in the FFPE samples was fair but not excellent (κ = 0.618). HPV DNA detection was highly significant, as measured by disease-specific survival and determined using a Wilcoxon test (P=.001). p16 IHC also exhibited a prognostic value but with a lower statistical significance (P=.0475). The detection of HPV DNA, but not p16 IHC, was also significantly correlated with locoregional control (P=.0461). CONCLUSION: Diagnostic methods based on the detection of HPV nucleic acids appear to be more reliable and objective because they do not require reading by a trained histopathologist. Furthermore, the detection of HPV DNA exhibits an improved correlation with survival, and therefore appears definitely more reliable than p16 IHC for routine use in clinical practice.
PURPOSE: Human papillomavirus (HPV) 16 infection is associated with oropharyngeal carcinogenesis and is likely the cause of the reported increase in disease incidence. We evaluated the prevalence of HPV infection and the reliability of different diagnostic tools using primary tumor samples from a cohort of 50 patients. METHODS AND MATERIALS: Formalin-fixed paraffin-embedded (FFPE) tumor samples were collected from all 50 consecutive primary oropharyngeal SCC patients who were enrolled in the study; fresh tumor samples were available in 22 cases. NucliSENS EasyQ HPVv1 was used for RNA, and Digene Hybrid Capture-2(HC2) was used for DNA detection. p16 Expression was evaluated by immunohistochemistry in FPPE specimens. RESULTS: Based on the DNA detection assay on FFPE samples, the frequency of high-risk HPV infection was 32%. The agreement rate between HPV RNA and HPV DNA detection in fresh samples was 100%. The agreement rate between p16 immunohistochemistry (IHC) and the detection of HPV DNA in the FFPE samples was fair but not excellent (κ = 0.618). HPV DNA detection was highly significant, as measured by disease-specific survival and determined using a Wilcoxon test (P=.001). p16 IHC also exhibited a prognostic value but with a lower statistical significance (P=.0475). The detection of HPV DNA, but not p16 IHC, was also significantly correlated with locoregional control (P=.0461). CONCLUSION: Diagnostic methods based on the detection of HPV nucleic acids appear to be more reliable and objective because they do not require reading by a trained histopathologist. Furthermore, the detection of HPV DNA exhibits an improved correlation with survival, and therefore appears definitely more reliable than p16 IHC for routine use in clinical practice.
Authors: Henrike Reder; Steffen Wagner; Nora Wuerdemann; Christine Langer; Sarah Sandmann; Andreas Braeuninger; Martin Dugas; Stefan Gattenloehner; Claus Wittekindt; Jens Peter Klussmann Journal: Cancer Med Date: 2021-02-01 Impact factor: 4.452
Authors: Roberto Gallus; Tarik Gheit; Dana Holzinger; Marco Petrillo; Davide Rizzo; Gianluigi Petrone; Francesco Miccichè; Gian Carlo Mattiucci; Damiano Arciuolo; Giampiero Capobianco; Giovanni Delogu; Vincenzo Valentini; Massimo Tommasino; Francesco Bussu Journal: Vaccines (Basel) Date: 2022-01-27
Authors: Luca Tagliaferri; Ashwini Budrukkar; Jacopo Lenkowicz; Mauricio Cambeiro; Francesco Bussu; Jose Luis Guinot; Guido Hildebrandt; Bengt Johansson; Jens E Meyer; Peter Niehoff; Angeles Rovirosa; Zoltán Takácsi-Nagy; Luca Boldrini; Nicola Dinapoli; Vito Lanzotti; Andrea Damiani; Roberto Gatta; Bruno Fionda; Valentina Lancellotta; Tamer Soror; Rafael Martìnez Monge; Vincenzo Valentini; György Kovács Journal: J Contemp Brachytherapy Date: 2018-06-30