Wei Zheng1, Lijian Niu2, Chunpu Zhang2, Chao Zhu2, Fangmin Xie2, Chunguang Cao2, Gang Li3. 1. Department of Neurosurgery, Qilu Hospital, Shandong University Jinan 250012, China ; Department of Neurosurgery, Affiliated Hospital of Taishan Medical University Tai'an 271000, China. 2. Department of Neurosurgery, Affiliated Hospital of Taishan Medical University Tai'an 271000, China. 3. Department of Neurosurgery, Qilu Hospital, Shandong University Jinan 250012, China.
Abstract
OBJECTIVE: To investigate brain edema and protein expression of c-Fos and c-Jun in brain after diffuse brain injury, and to investigate the pathological change after brain injury, which may provide evidence for the clinical treatment of diffused brain injury. METHODS: Marmarou method was used to establish the diffuse brain injury in rats. RESULTS: After diffused brain injury, brain water content increased at 1 h, reached the peak at 1 d and remained at a high level at 7 d when compared with control group. One day after injury, diffuse subarachnoid hemorrhage was observed in the brain. HE staining showed vascular swelling and bleeding at the cortex and corpus callosum at 1 d. β-APP expression was found at the brainstem, hippocampus, thalamus, corpus callosum and periventricular regions. Pathological examination of ultrathin sections showed evidence edema and fracture of axons at 3 d after brain injury. The brain injury caused severe cerebral ischemia. The c-Fos and c-Jun expression increased at 1 h. The c-Fos expression peaked at 3 h (P < 0.05), then reduced, reached a maximal level again at 3 d (P < 0.05), and reduced significantly at 7 d but remained at a higher level when compared with control group (P < 0.05). The number of c-Jun positive cells peaked at 6 h (P < 0.05), then reduced, reached a maximal level again at 3 d and reduced markedly but still remained at a higher level when compared with control group (P < 0.05). CONCLUSION: After diffuse brain injury, brain water content and c-Fos/c-Jun expression change over time.
OBJECTIVE: To investigate brain edema and protein expression of c-Fos and c-Jun in brain after diffuse brain injury, and to investigate the pathological change after brain injury, which may provide evidence for the clinical treatment of diffused brain injury. METHODS: Marmarou method was used to establish the diffuse brain injury in rats. RESULTS: After diffused brain injury, brain water content increased at 1 h, reached the peak at 1 d and remained at a high level at 7 d when compared with control group. One day after injury, diffuse subarachnoid hemorrhage was observed in the brain. HE staining showed vascular swelling and bleeding at the cortex and corpus callosum at 1 d. β-APP expression was found at the brainstem, hippocampus, thalamus, corpus callosum and periventricular regions. Pathological examination of ultrathin sections showed evidence edema and fracture of axons at 3 d after brain injury. The brain injury caused severe cerebral ischemia. The c-Fos and c-Jun expression increased at 1 h. The c-Fos expression peaked at 3 h (P < 0.05), then reduced, reached a maximal level again at 3 d (P < 0.05), and reduced significantly at 7 d but remained at a higher level when compared with control group (P < 0.05). The number of c-Jun positive cells peaked at 6 h (P < 0.05), then reduced, reached a maximal level again at 3 d and reduced markedly but still remained at a higher level when compared with control group (P < 0.05). CONCLUSION: After diffuse brain injury, brain water content and c-Fos/c-Jun expression change over time.
Authors: Joost W Schouten; Carl T Fulp; Nicolas C Royo; Kathryn E Saatman; Deborah J Watson; Evan Y Snyder; John Q Trojanowski; Darwin J Prockop; Andrew I R Maas; Tracy K McIntosh Journal: J Neurotrauma Date: 2004-11 Impact factor: 5.269
Authors: Selim Çomaklı; Çiğdem Sevim; George Kontadakis; Elif Doğan; Ali Taghizadehghalehjoughi; Mustafa Özkaraca; Michael Aschner; Taxiarchis Konstantinos Nikolouzakis; Aristides Tsatsakis Journal: Toxicol Rep Date: 2019-01-16