| Literature DB >> 25030930 |
Abstract
The ability to purify an intact, functional protein or protein complex is an essential step in biochemical characterization studies. Challenges in purification arise when proteins are of low abundance or are unstable resulting in low yields or poor in vitro activity. In this protocol we describe a method to purify active, recombinant human proteins fused to a tandem MBP tag after expression in human 293T cells.Entities:
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Year: 2014 PMID： 25030930 DOI： 10.1007/978-1-4939-0992-6_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745