Literature DB >> 25030770

Comparison of the substrate selectivity and biochemical properties of human and bacterial γ-butyrobetaine hydroxylase.

Anna M Rydzik1, Ivanhoe K H Leung, Grazyna T Kochan, Nikita D Loik, Luc Henry, Michael A McDonough, Timothy D W Claridge, Christopher J Schofield.   

Abstract

2-Oxoglutarate and iron dependent oxygenases have potential for the stereoselective hydroxylation of amino acids and related compounds. The biochemical and kinetic properties of recombinant γ-butyrobetaine hydroxylase from human and Pseudomonas sp. AK1 were compared. The results reveal differences between the two BBOXs, including in their stimulation by ascorbate. Despite their closely related sequences, the two enzymes also display different substrate selectivities, including for the production of (di)hydroxylated betaines, implying use of engineered BBOXs for biocatalytic purposes may be productive.

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Year:  2014        PMID： 25030770     DOI： 10.1039/c4ob01167h

Source DB:  PubMed          Journal:  Org Biomol Chem        ISSN： 1477-0520            Impact factor:   3.876


  7 in total

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