Effect of stress conditions on Escherichia coli outer and inner membranes, separated by Sephacryl S-500 chromatography.

A chromatographic method for the separation of outer and inner membranes of Escherichia coli is described. This method is much faster than the generally used equilibrium centrifugation and has a greater versatility in the volumes that can be treated. Both techniques are used to examine the effects of different stress conditions on the E. coli membrane. The obtained results illustrate the equivalence of both methods.