| Literature DB >> 25019997 |
Zhangming Chen1, Mengying Zhu1, Xiang Pan1, Yongji Zhu1, Hai Yan1, Tongcui Jiang2, Yujun Shen2, Xiaowan Dong1, Nan Zheng1, Jinsen Lu1, Songcheng Ying3, Yuxian Shen4.
Abstract
Sterile alpha motif and HD-domain containing protein 1 (SAMHD1) is a newly identified intracellular antiviral factor. By depleting the dNTPs pool of host cells to a low level that cannot support the efficient synthesis of viral cDNA, it restricts replication of some retroviruses. As a DNA virus, Hepatitis B virus (HBV) experiences a process of reverse transcription in its life cycle akin to that of retroviruses. However, whether SAMHD1 can restrict HBV replication in liver cells is unknown. Here, we reported that SAMHD1 expression was detectable in four liver cell lines. Exogenous expression of SAMHD1 in SMMC-7721 cells restrained HBV replication. Similarly, SAMHD1 impeded HBV replication in another liver cell line, BEL-7402. Remarkably, the catalytically inactive mutant, SAMHD1 HD/AA also hampered HBV replication. Additionally, HBV replication reduced SAMHD1 expression in HepG2 cells. Moreover, it was found that IFN-α induced expression of SAMHD1 in liver cells. Together, these findings suggested that IFN-α-inducible SAMHD1 inhibited HBV replication in liver cells.Entities:
Keywords: HBV; IFN-α; Liver cell; SAMHD1
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Year: 2014 PMID： 25019997 DOI： 10.1016/j.bbrc.2014.07.023
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575