BACKGROUND: To understand the function of β6 integrin and elucidate its signaling pathways in TGF-β-induced EMT in breast cancer. METHODS: The interactions between TGF-β1 and β6 integrin were measured by coimmunoprecipitation. The EMT responses, phospherlation of PI3K/Akt and COX-2 expression were determined by real-time PCR, transwell assay, and western blot after the blockage of β6 integrin. RESULTS: TGF-β1 and β6 integrin could bind with each other. Blockage of β6 integrin rescued TGF-β1-induced EMT phenotype and reduced expression of COX-2 via dephosphorylation of PI3K/Akt. CONCLUSIONS: β6 integrin plays a critical role in TGF-β1-induced EMT and overexpression of COX-2 in breast cancer.
BACKGROUND: To understand the function of β6 integrin and elucidate its signaling pathways in TGF-β-induced EMT in breast cancer. METHODS: The interactions between TGF-β1 and β6 integrin were measured by coimmunoprecipitation. The EMT responses, phospherlation of PI3K/Akt and COX-2 expression were determined by real-time PCR, transwell assay, and western blot after the blockage of β6 integrin. RESULTS: TGF-β1 and β6 integrin could bind with each other. Blockage of β6 integrin rescued TGF-β1-induced EMT phenotype and reduced expression of COX-2 via dephosphorylation of PI3K/Akt. CONCLUSIONS: β6 integrin plays a critical role in TGF-β1-induced EMT and overexpression of COX-2 in breast cancer.
Entities:
Keywords:
Breast carcinoma; COX-2; EMT; PI3K pathways; TGF-β; β6 integrin
Authors: Abbas K Samadi; Alan Bilsland; Alexandros G Georgakilas; Amedeo Amedei; Amr Amin; Anupam Bishayee; Asfar S Azmi; Bal L Lokeshwar; Brendan Grue; Carolina Panis; Chandra S Boosani; Deepak Poudyal; Diana M Stafforini; Dipita Bhakta; Elena Niccolai; Gunjan Guha; H P Vasantha Rupasinghe; Hiromasa Fujii; Kanya Honoki; Kapil Mehta; Katia Aquilano; Leroy Lowe; Lorne J Hofseth; Luigi Ricciardiello; Maria Rosa Ciriolo; Neetu Singh; Richard L Whelan; Rupesh Chaturvedi; S Salman Ashraf; H M C Shantha Kumara; Somaira Nowsheen; Sulma I Mohammed; W Nicol Keith; William G Helferich; Xujuan Yang Journal: Semin Cancer Biol Date: 2015-05-05 Impact factor: 15.707
Authors: Marta Dyszkiewicz-Konwińska; Mariusz J Nawrocki; Yan Huang; Artur Bryja; Piotr Celichowski; Maurycy Jankowski; Katarzyna Błochowiak; Katarzyna Mehr; Małgorzata Bruska; Michał Nowicki; Maciej Zabel; Bartosz Kempisty Journal: Int J Mol Sci Date: 2018-03-29 Impact factor: 5.923