Amirhossein Fereidunian1, Mona Sadeghalvad1, Maryam Omidi Oscoie1, Ali Mostafaie2. 1. Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. 2. Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran; Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic address: amostafaie@kums.ac.ir.
Abstract
BACKGROUND AND AIMS: Bowman-Birk protease inhibitor (BBI) has been well known to suppress the emergence and progression of different cancers. In the present study, the mechanisms by which BBI alters cancers have been addressed. To reach this goal, the effects of BBI on proliferation of and VEGF secretion by two cell lines (AGS: gastric adenocarcinoma and HT-29: colorectal adenocarcinoma) and also BBI effect on MMP-2 and 9 synthesis/secretion by AGS cells was evaluated. METHODS: ELISA method was used to assess VEGF concentration and gelatin zymography was used to address MMP-2 and 9 production/excretion. RESULTS: BBI had powerful inhibitory effect on proliferation and VEGF secretion by both cell lines. In addition, inhibition of MMP-2 and MMP-9 secreted by AGS cells suggests BBI as a potent inhibitor of gastric cancer progression. On the other hand, the results indicated that inhibition of MMP-2, MMP-9 and VEGF secretion is one of the mechanisms of anti-angiogenic effect of BBI. CONCLUSION: BBI expresses powerful suppressive effect on tumor progression of two prevalent cancers: gastric adenocarcinoma and colorectal adenocarcinoma.
BACKGROUND AND AIMS: Bowman-Birk protease inhibitor (BBI) has been well known to suppress the emergence and progression of different cancers. In the present study, the mechanisms by which BBI alters cancers have been addressed. To reach this goal, the effects of BBI on proliferation of and VEGF secretion by two cell lines (AGS: gastric adenocarcinoma and HT-29: colorectal adenocarcinoma) and also BBI effect on MMP-2 and 9 synthesis/secretion by AGS cells was evaluated. METHODS: ELISA method was used to assess VEGF concentration and gelatin zymography was used to address MMP-2 and 9 production/excretion. RESULTS:BBI had powerful inhibitory effect on proliferation and VEGF secretion by both cell lines. In addition, inhibition of MMP-2 and MMP-9 secreted by AGS cells suggests BBI as a potent inhibitor of gastric cancer progression. On the other hand, the results indicated that inhibition of MMP-2, MMP-9 and VEGF secretion is one of the mechanisms of anti-angiogenic effect of BBI. CONCLUSION:BBI expresses powerful suppressive effect on tumor progression of two prevalent cancers: gastric adenocarcinoma and colorectal adenocarcinoma.
Authors: Martín Indarte; Cristian M Lazza; Diego Assis; Néstor O Caffini; María A Juliano; Francesc X Avilés; Xavier Daura; Laura M I López; Sebastián A Trejo Journal: Planta Date: 2016-10-24 Impact factor: 4.116
Authors: Ayami Sato; Ivone Izabel Mackowiak da Fonseca; Márcia Kazumi Nagamine; Gabriela Fernandes de Toledo; Rennan Olio; Francisco Javier Hernandez-Blazquez; Tomohiro Yano; Elizabeth Shinmay Yeh; Maria Lucia Zaidan Dagli Journal: Front Vet Sci Date: 2021-06-10