Literature DB >> 25013081

Nucleic acid amplification tests for the diagnosis of Neisseria gonorrhoeae in low-prevalence settings: a review of the evidence.

Helen Fifer1, Catherine A Ison1.   

Abstract

OBJECTIVES: In UK Microbiology laboratories there is widespread use of nucleic acid amplification tests (NAATs) which allow the simultaneous 'dual' detection of Neisseria gonorrhoeae and Chlamydia trachomatis, although the prevalence of gonorrhoea in most areas is low and this may lead to high numbers of false positive results. The aim of this study was to examine the evidence base for unselected testing for gonorrhoea in the community.
METHODS: A literature search was performed to review the use of dual testing in low prevalence settings by searching PubMed for appropriate terms linked to gonorrhoea diagnosis up to 1 December 2013 but without restriction of publication date. All publications with a prevalence of <1% were defined as low prevalence and included in this review.
RESULTS: The publication search found data in low prevalence settings from three sources; genitourinary medicine clinics, laboratories outside the UK and from the National Chlamydia Screening Programme. The evidence base to support widespread screening for gonorrhoea was found to be limited and of variable quality.
CONCLUSIONS: We were unable to find an evidence base to support widespread screening for gonorrhoea in the community. However, the increasing availability of dual NAATs may lead to more testing but this should be tempered by the public health need. Pilot studies and development of robust testing algorithms should be encouraged. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Entities:  

Keywords:  DNA AMPLIFICATION; GONORRHOEA; MOLECULAR TECHNIQUES; TESTING

Mesh:

Year:  2014        PMID: 25013081     DOI: 10.1136/sextrans-2014-051588

Source DB:  PubMed          Journal:  Sex Transm Infect        ISSN: 1368-4973            Impact factor:   3.519


  3 in total

1.  Evaluation of the New BD Max GC Real-Time PCR Assay, Analytically and Clinically as a Supplementary Test for the BD ProbeTec GC Qx Amplified DNA Assay, for Molecular Detection of Neisseria gonorrhoeae.

Authors:  Daniel Golparian; Stina Boräng; Martin Sundqvist; Magnus Unemo
Journal:  J Clin Microbiol       Date:  2015-10-14       Impact factor: 5.948

2.  Confirmatory assays are essential when using molecular testing for Neisseria gonorrhoeae in low-prevalence settings: insights from the third National Survey of Sexual Attitudes and Lifestyles (Natsal-3).

Authors:  Nigel Field; Soazig Clifton; Sarah Alexander; Catherine A Ison; Gwenda Hughes; Simon Beddows; Clare Tanton; Kate Soldan; Filomeno Coelho da Silva; Catherine H Mercer; Kaye Wellings; Anne M Johnson; Pam Sonnenberg
Journal:  Sex Transm Infect       Date:  2014-12-15       Impact factor: 3.519

3.  Screening for gonorrhoea using samples collected through the English national chlamydia screening programme and risk of false positives: a national survey of local authorities.

Authors:  Nigel Field; Iain Kennedy; Kate Folkard; Stephen Duffell; Katy Town; Catherine A Ison; Gwenda Hughes
Journal:  BMJ Open       Date:  2014-10-16       Impact factor: 2.692

  3 in total

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