Takaharu Kozakai1, Mitsue Sakate2, Satoshi Takizawa3, Tsuyoshi Uchide4, Hisato Kobayashi5, Katsutaka Oishi6, Norio Ishida7, Kaname Saida8. 1. Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan; Yamagata University, Faculty of Education, Art and Science, Kojirakawa 1-4-12, Yamagata 990-8560, Japan. 2. International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan. 3. Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan. 4. Veterinary Internal Medicine, Department of Small Animal Clinical Sciences, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. 5. Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan. 6. Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan; Institute for Biomedical Research, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan. 7. Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan; Institute for Biomedical Research, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan. 8. Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan; International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan; Institute for Biomedical Research, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan; Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka 563-8577, Japan. Electronic address: k.saida@aist.go.jp.
Abstract
AIMS: The function, regulation and gene expression of the endothelin (ET) system in the intestine is not well understood. We investigated the dependence on feeding schedule and biological clock of the regulation of ET-1 gene expression in mouse colon. MAIN METHODS: Mice were fed freely, fasted for 48 h and re-fed after fasting. KEY FINDINGS: Where indicated ET-1 gene expression was highest in the colon compared with other tissues examined in fasted mice. Fasting increased the level, while maintaining the rhythmicity, of ET-1 gene expression in epithelial colonic tissue. Re-feeding, however, decreased ET-1 gene expression and suppressed rhythmic oscillation, and the rhythmicity also changed for gene expression for circadian clocks, period-1 and period-2 (Per1 and Per2). Furthermore, the decrease in ET-1 gene expression induced by re-feeding was blocked by pre-treatment with hexamethonium and atropine. The daily change in ET-1 gene expression in colon, which depends on feeding schedule via the autonomic nervous system, is synchronized with peripheral circadian oscillators under conditions of free feeding and fasting but not re-feeding. The decrease in ET-1 gene expression in the proximal colon induced by re-feeding occurs via the nervous system. SIGNIFICANCE: ET-1 plays an important physiological role, which is dependent on feeding behavior.
AIMS: The function, regulation and gene expression of the endothelin (ET) system in the intestine is not well understood. We investigated the dependence on feeding schedule and biological clock of the regulation of ET-1 gene expression in mouse colon. MAIN METHODS:Mice were fed freely, fasted for 48 h and re-fed after fasting. KEY FINDINGS: Where indicated ET-1 gene expression was highest in the colon compared with other tissues examined in fasted mice. Fasting increased the level, while maintaining the rhythmicity, of ET-1 gene expression in epithelial colonic tissue. Re-feeding, however, decreased ET-1 gene expression and suppressed rhythmic oscillation, and the rhythmicity also changed for gene expression for circadian clocks, period-1 and period-2 (Per1 and Per2). Furthermore, the decrease in ET-1 gene expression induced by re-feeding was blocked by pre-treatment with hexamethonium and atropine. The daily change in ET-1 gene expression in colon, which depends on feeding schedule via the autonomic nervous system, is synchronized with peripheral circadian oscillators under conditions of free feeding and fasting but not re-feeding. The decrease in ET-1 gene expression in the proximal colon induced by re-feeding occurs via the nervous system. SIGNIFICANCE: ET-1 plays an important physiological role, which is dependent on feeding behavior.
Authors: Alexander Dueck; Christoph Berger; Katharina Wunsch; Johannes Thome; Stefan Cohrs; Olaf Reis; Frank Haessler Journal: J Neural Transm (Vienna) Date: 2015-10-15 Impact factor: 3.575
Authors: Lauren G Douma; Kristen Solocinski; Sarah H Masten; Dominique H Barral; Sarah J Barilovits; Lauren A Jeffers; Kareme D Alder; Ravi Patel; Charles S Wingo; Kevin D Brown; Brian D Cain; Michelle L Gumz Journal: Front Physiol Date: 2020-03-13 Impact factor: 4.566