M Salehipour1, K Izadpanah2, A Safaei3, M Kamranpoor4, M R Farsiani4. 1. Department of Urology, Shahid Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Department of Urology, Fasa University of Medical Sciences, Fars, Iran. 3. Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
PURPOSES: To evaluate the efficacy of human amniotic membrane (AM) grafting in the canine penile tunica albuginea defect; we developed an animal model as the first step toward an innovating new method for the treatment of Peyronie's disease, penile cancers, and congenital deformities of the penis. MATERIAL AND METHODS: From August to September 2011, ten healthy male dogs were selected. A rhomboid incision about 3 x 2 cm over the tunica albuginea and its overlying squamous epithelium was made and then excised. The amniotic membrane was folded twice on itself and grafted on the defect. After 8 weeks, artificial erection was made for 5 dogs and for the other 5 dogs after 12 weeks. After artificial erection, partial penectomy was done and histopathological evaluation was performed on the grafts. RESULTS: Artificial erection performed successfully in all of the dogs. No infection or any other complication was seen. Histopathological examination showed complete re-epithelialization with squamous epithelium and collagen fiber deposition. Also, no dysplasia was seen. CONCLUSIONS: The amniotic membrane can be used as a suitable substitution for tunica albuginea. It is safe, inexpensive, biodegradable, and available and may be used for the treatment of Peyronie ' s disease, penile cancers, congenital penile deformities, and penile reconstructive surgery.
PURPOSES: To evaluate the efficacy of human amniotic membrane (AM) grafting in the caninepenile tunica albuginea defect; we developed an animal model as the first step toward an innovating new method for the treatment of Peyronie's disease, penile cancers, and congenital deformities of the penis. MATERIAL AND METHODS: From August to September 2011, ten healthy male dogs were selected. A rhomboid incision about 3 x 2 cm over the tunica albuginea and its overlying squamous epithelium was made and then excised. The amniotic membrane was folded twice on itself and grafted on the defect. After 8 weeks, artificial erection was made for 5 dogs and for the other 5 dogs after 12 weeks. After artificial erection, partial penectomy was done and histopathological evaluation was performed on the grafts. RESULTS: Artificial erection performed successfully in all of the dogs. No infection or any other complication was seen. Histopathological examination showed complete re-epithelialization with squamous epithelium and collagen fiber deposition. Also, no dysplasia was seen. CONCLUSIONS: The amniotic membrane can be used as a suitable substitution for tunica albuginea. It is safe, inexpensive, biodegradable, and available and may be used for the treatment of Peyronie ' s disease, penile cancers, congenital penile deformities, and penile reconstructive surgery.