Literature DB >> 25005105

Crystallization and preliminary analysis of the NqrA and NqrC subunits of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae.

Georg Vohl1, Ruslan Nedielkov2, Björn Claussen3, Marco S Casutt1, Thomas Vorburger4, Kay Diederichs2, Heiko M Möller2, Julia Steuber4, Günter Fritz1.   

Abstract

The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio cholerae is a membrane protein complex consisting of six different subunits NqrA-NqrF. The major domains of the NqrA and NqrC subunits were heterologously expressed in Escherichia coli and crystallized. The structure of NqrA1-377 was solved in space groups C222₁ and P2₁ by SAD phasing and molecular replacement at 1.9 and 2.1 Å resolution, respectively. NqrC devoid of the transmembrane helix was co-expressed with ApbE to insert the flavin mononucleotide group covalently attached to Thr225. The structure was determined by molecular replacement using apo-NqrC of Parabacteroides distasonis as search model at 1.8 Å resolution.

Entities:  

Keywords:  Na+-translocating NQR; Vibrio cholerae; covalently bound FMN

Mesh：

Substances：

Year:  2014        PMID： 25005105      PMCID： PMC4089548          DOI： 10.1107/S2053230X14009881

Source DB:  PubMed          Journal:  Acta Crystallogr F Struct Biol Commun        ISSN： 2053-230X            Impact factor:   1.056


  24 in total

1.  Crystallization of the Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae.

Authors:  Marco S Casutt; Severin Wendelspiess; Julia Steuber; Günter Fritz
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-11-27

2.  Protein structure prediction on the Web: a case study using the Phyre server.

Authors:  Lawrence A Kelley; Michael J E Sternberg
Journal:  Nat Protoc       Date:  2009       Impact factor: 13.491

3.  Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7.

Authors:  Gerrit Langer; Serge X Cohen; Victor S Lamzin; Anastassis Perrakis
Journal:  Nat Protoc       Date:  2008       Impact factor: 13.491

4.  Localization of ubiquinone-8 in the Na+-pumping NADH:quinone oxidoreductase from Vibrio cholerae.

Authors:  Marco S Casutt; Ruslan Nedielkov; Severin Wendelspiess; Sara Vossler; Uwe Gerken; Masatoshi Murai; Hideto Miyoshi; Heiko M Möller; Julia Steuber
Journal:  J Biol Chem       Date:  2011-09-01       Impact factor: 5.157

5.  The single NqrB and NqrC subunits in the Na(+)-translocating NADH: quinone oxidoreductase (Na(+)-NQR) from Vibrio cholerae each carry one covalently attached FMN.

Authors:  Marco S Casutt; Andreas Schlosser; Wolfgang Buckel; Julia Steuber
Journal:  Biochim Biophys Acta       Date:  2012-02-16

6.  Linking crystallographic model and data quality.

Authors:  P Andrew Karplus; Kay Diederichs
Journal:  Science       Date:  2012-05-25       Impact factor: 47.728

7.  Membrane topology mapping of the Na+-pumping NADH: quinone oxidoreductase from Vibrio cholerae by PhoA-green fluorescent protein fusion analysis.

Authors:  Ellen B Duffy; Blanca Barquera
Journal:  J Bacteriol       Date:  2006-10-13       Impact factor: 3.490

8.  Alternative pyrimidine biosynthesis protein ApbE is a flavin transferase catalyzing covalent attachment of FMN to a threonine residue in bacterial flavoproteins.

Authors:  Yulia V Bertsova; Maria S Fadeeva; Vitaly A Kostyrko; Marina V Serebryakova; Alexander A Baykov; Alexander V Bogachev
Journal:  J Biol Chem       Date:  2013-04-04       Impact factor: 5.157

9.  The Buccaneer software for automated model building. 1. Tracing protein chains.

Authors:  Kevin Cowtan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2006-08-19

10.  Phaser crystallographic software.

Authors:  Airlie J McCoy; Ralf W Grosse-Kunstleve; Paul D Adams; Martyn D Winn; Laurent C Storoni; Randy J Read
Journal:  J Appl Crystallogr       Date:  2007-07-13       Impact factor: 3.304
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  7 in total

1.  Structure of the V. cholerae Na+-pumping NADH:quinone oxidoreductase.

Authors:  Julia Steuber; Georg Vohl; Marco S Casutt; Thomas Vorburger; Kay Diederichs; Günter Fritz
Journal:  Nature       Date:  2014-12-04       Impact factor: 49.962

2.  Structural and functional investigation of flavin binding center of the NqrC subunit of sodium-translocating NADH:quinone oxidoreductase from Vibrio harveyi.

Authors:  Valentin Borshchevskiy; Ekaterina Round; Yulia Bertsova; Vitaly Polovinkin; Ivan Gushchin; Andrii Ishchenko; Kirill Kovalev; Alexey Mishin; Galina Kachalova; Alexander Popov; Alexander Bogachev; Valentin Gordeliy
Journal:  PLoS One       Date:  2015-03-03       Impact factor: 3.240

3.  Vibrio natriegens as Host for Expression of Multisubunit Membrane Protein Complexes.

Authors:  Lena Schleicher; Valentin Muras; Björn Claussen; Jens Pfannstiel; Bastian Blombach; Pavel Dibrov; Günter Fritz; Julia Steuber
Journal:  Front Microbiol       Date:  2018-10-25       Impact factor: 5.640

4.  Molecular dynamics modeling of the Vibrio cholera Na+-translocating NADH:quinone oxidoreductase NqrB-NqrD subunit interface.

Authors:  Alexander Dibrov; Muntahi Mourin; Pavel Dibrov; Grant N Pierce
Journal:  Mol Cell Biochem       Date:  2021-10-09       Impact factor: 3.396

5.  A Sodium-Translocating Module Linking Succinate Production to Formation of Membrane Potential in Prevotella bryantii.

Authors:  Lena Schleicher; Andrej Trautmann; Dennis P Stegmann; Günter Fritz; Jochem Gätgens; Michael Bott; Sascha Hein; Jörg Simon; Jana Seifert; Julia Steuber
Journal:  Appl Environ Microbiol       Date:  2021-09-01       Impact factor: 4.792

6.  Central Carbon Metabolism, Sodium-Motive Electron Transfer, and Ammonium Formation by the Vaginal Pathogen Prevotella bivia.

Authors:  Lena Schleicher; Sebastian Herdan; Günter Fritz; Andrej Trautmann; Jana Seifert; Julia Steuber
Journal:  Int J Mol Sci       Date:  2021-11-03       Impact factor: 5.923

7.  The Membrane-Bound C Subunit of Reductive Dehalogenases: Topology Analysis and Reconstitution of the FMN-Binding Domain of PceC.

Authors:  Géraldine F Buttet; Mathilde S Willemin; Romain Hamelin; Aamani Rupakula; Julien Maillard
Journal:  Front Microbiol       Date:  2018-04-24       Impact factor: 5.640
  7 in total

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