Literature DB >> 2499882

tRNA-tRNA interactions within cellular ribosomes.

D Smith1, M Yarus.   

Abstract

We describe an assay that converts the effects of tRNA-tRNA contacts at two particular codons into a quantitative effect on beta-galactosidase level. The assay measures the separate and combined efficiency of suppression at adjacent nonsense codons in vivo using a set of specially created homologous messages. In a survey of distal anticodon arm substitutions, we find that particular mutant tRNAs occupying the P-site reduce the apparent efficiency of the suppressor tRNA reading the A-site codon by factors of 2-170. By using measured tRNA-tRNA distances and the crystallographic tRNA structure, we propose a model of the tRNA-tRNA-mRNA complex. In the model, the anticodon loops of the P-site and A-site tRNAs contact one another in a way that is consistent with our combined tRNA efficiency data. These results suggest that tRNA-tRNA interactions that modulate tRNA action are an inevitable feature of translation.

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Year:  1989        PMID： 2499882      PMCID： PMC287276          DOI： 10.1073/pnas.86.12.4397

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  17 in total

1.  A stereochemical model of the transpeptidation complex.

Authors:  J J McDonald; R Rein
Journal:  J Biomol Struct Dyn       Date:  1987-04

2.  The distance between the anticodon loops of two tRNAs bound to the 70 S Escherichia coli ribosome.

Authors:  R H Fairclough; C R Cantor
Journal:  J Mol Biol       Date:  1979-08-25       Impact factor: 5.469

3.  Molecular mechanics of translation: a reciprocating ratchet mechanism.

Authors:  C Woese
Journal:  Nature       Date:  1970-05-30       Impact factor: 49.962

4.  Interaction between RNA1 and the primer precursor in the regulation of Co1E1 replication.

Authors:  R M Lacatena; G Cesareni
Journal:  J Mol Biol       Date:  1983-11-05       Impact factor: 5.469

5.  Context effects: translation of UAG codon by suppressor tRNA is affected by the sequence following UAG in the message.

Authors:  L Bossi
Journal:  J Mol Biol       Date:  1983-02-15       Impact factor: 5.469

6.  Effects of surrounding sequence on the suppression of nonsense codons.

Authors:  J H Miller; A M Albertini
Journal:  J Mol Biol       Date:  1983-02-15       Impact factor: 5.469

7.  Distance moved by transfer RNA during translocation from the A site to the P site on the ribosome.

Authors:  A E Johnson; H J Adkins; E A Matthews; C R Cantor
Journal:  J Mol Biol       Date:  1982-03-25       Impact factor: 5.469

8.  Codon context effects in missense suppression.

Authors:  E J Murgola; F T Pagel; K A Hijazi
Journal:  J Mol Biol       Date:  1984-05-05       Impact factor: 5.469

9.  Topological arrangement of two transfer RNAs on the ribosome. Fluorescence energy transfer measurements between A and P site-bound tRNAphe.

Authors:  H Paulsen; J M Robertson; W Wintermeyer
Journal:  J Mol Biol       Date:  1983-06-25       Impact factor: 5.469

10.  Translational efficiency of transfer RNA's: uses of an extended anticodon.

Authors:  M Yarus
Journal:  Science       Date:  1982-11-12       Impact factor: 47.728
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  43 in total

1.  Analysis of codon:anticodon interactions within the ribosome provides new insights into codon reading and the genetic code structure.

Authors:  V I Lim; J F Curran
Journal:  RNA       Date:  2001-07       Impact factor: 4.942

Review 2.  Suppression and the code: beyond codons and anticodons.

Authors:  E J Murgola
Journal:  Experientia       Date:  1990-12-01

3.  Kinetic basis for global loss of fidelity arising from mismatches in the P-site codon:anticodon helix.

Authors:  Hani S Zaher; Rachel Green
Journal:  RNA       Date:  2010-08-19       Impact factor: 4.942

4.  How are tRNAs and mRNA arranged in the ribosome? An attempt to correlate the stereochemistry of the tRNA-mRNA interaction with constraints imposed by the ribosomal topography.

Authors:  V Lim; C Venclovas; A Spirin; R Brimacombe; P Mitchell; F Müller
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

5.  Control of translation efficiency in yeast by codon-anticodon interactions.

Authors:  Daniel P Letzring; Kimberly M Dean; Elizabeth J Grayhack
Journal:  RNA       Date:  2010-10-22       Impact factor: 4.942

6.  A self-referential model for the formation of the genetic code.

Authors:  Romeu Cardoso Guimarães; Carlos Henrique Costa Moreira; Sávio Torres de Farias
Journal:  Theory Biosci       Date:  2008-05-21       Impact factor: 1.919

7.  Decoding with the A:I wobble pair is inefficient.

Authors:  J F Curran
Journal:  Nucleic Acids Res       Date:  1995-02-25       Impact factor: 16.971

8.  UGA suppression by tRNACmCATrp occurs in diverse virus RNAs due to a limited influence of the codon context.

Authors:  C Urban; K Zerfass; C Fingerhut; H Beier
Journal:  Nucleic Acids Res       Date:  1996-09-01       Impact factor: 16.971

9.  Special peptidyl-tRNA molecules can promote translational frameshifting without slippage.

Authors:  A Vimaladithan; P J Farabaugh
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272

10.  An anticodon sequence mutant of Escherichia coli initiator tRNA: possible importance of a newly acquired base modification next to the anticodon on its activity in initiation.

Authors:  D Mangroo; P A Limbach; J A McCloskey; U L RajBhandary
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490
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