Y Fang1, S Zeng1, X Fu1, B Jia1, S Li2, X An3, Y Chen3, S Zhu1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. 2. Bingjing AnBo Embryo Biotech Center, Beijing, China. 3. College of Biological Sciences, China Agricultural University, Beijing, China.
Abstract
BACKGROUND: It is uncertain whether long-term cryopreservation affects embryonic development. OBJECTIVE: This study was to investigate the effects of long-term cryopreservation on in vitro and in vivo developmental competence of bovine blastocysts. METHODS: The blastocysts were randomly allocated into 3 groups based on the storage time: 0.5-year group, 1-year group and 15-years group. The thawed blastocysts were subjected to in vitro culture or embryo transplantation. RESULT: Significantly lower survival rate (89.2%) and re-expansion rate (70.3%) of blastocysts were obtained from 15-years group compared with those of 0.5-year (97.5% and 87.5%) and 1-year (100% and 84.2%) groups (P < 0.05). There were no significant differences in the hatching rate (39.5% to 42.5%) among the three groups and the pregnancy rate between 1-year (35.0%) and 15-years (36.4%) groups. CONCLUSIONS: Although in vitro developmental competence of the 15 years cryopreserved blastocysts was decreased slightly, the pregnancy outcome was not affected.
RCT Entities:
BACKGROUND: It is uncertain whether long-term cryopreservation affects embryonic development. OBJECTIVE: This study was to investigate the effects of long-term cryopreservation on in vitro and in vivo developmental competence of bovineblastocysts. METHODS: The blastocysts were randomly allocated into 3 groups based on the storage time: 0.5-year group, 1-year group and 15-years group. The thawed blastocysts were subjected to in vitro culture or embryo transplantation. RESULT: Significantly lower survival rate (89.2%) and re-expansion rate (70.3%) of blastocysts were obtained from 15-years group compared with those of 0.5-year (97.5% and 87.5%) and 1-year (100% and 84.2%) groups (P < 0.05). There were no significant differences in the hatching rate (39.5% to 42.5%) among the three groups and the pregnancy rate between 1-year (35.0%) and 15-years (36.4%) groups. CONCLUSIONS: Although in vitro developmental competence of the 15 years cryopreserved blastocysts was decreased slightly, the pregnancy outcome was not affected.