B Zevallos1, I Cejas2, F Engelmann3, D Carputo4, R Aversano5, M T Scarano6, E Yanes7, M Martinez-Montero7, J C Lorenzo7. 1. Escuela Superior Politecnica Agropecuaria de Manabi Manuel Felix Lopez (ESPAM), Campus Politecnico El Limon, Carrera de Ingenieria Agricola, Calceta, Manabi, Ecuador. bzevallos@espam.edu.ec. 2. Faculty of Agronomy, Universidad de Ciego de Avila, Ciego de Avila 69450, Cuba. 3. IRD, UMR DIADE, Montpellier Cedex, France. 4. Department of Agricultural Sciences, University of Naples Federico II, Via Universita, Portici (NA), Italy. 5. Department of Agricultural Sciences, University of Naples Federico II, Via Universita 100 - 80055 Portici (NA), Italy. 6. Institute of Plant Genetics (Research Division Portici), National Council of Research, Via Universita, Portici (NA), Italy. 7. Laboratory for Plant Breeding, Centro de Bioplantas, Universidad de Ciego de Avila, Ciego de Avila 69450, Cuba.
Abstract
BACKGROUND: Before cryopreservation is routinely used, its effect on the trueness-to-type of the regenerated plant material needs to be evaluated. OBJECTIVE: In this work, we studied the effect of seed cryopreservation on the phenotypic and molecular characteristics of wild Solanum lycopersicum Mill. plants. METHODS: Thirty-five morphological traits of plants regenerated from cryopreserved seeds were compared to those measured on plants regenerated from non-cryopreserved seeds. RESULT: No statistically significant differences were observed between cryopreserved and non-cryopreserved samples, either in the first or in the second generation post-liquid nitrogen exposure. However, at the molecular level, the genetic analyses performed on the second generation plants germinated from control and cryopreserved seeds using 14 nuclear Simple Sequences Repeats (SSR) markers uncovered some changes in microsatellite length between control and cryopreserved samples. These results confirm at the botanical phenotype level the effectiveness of seed cryostorage for conservation and regeneration of true-to-type S. lycopersicum plants. CONCLUSION: Further experiments are required to clarify potential phenotypic effects of the changes observed in the DNA.
BACKGROUND: Before cryopreservation is routinely used, its effect on the trueness-to-type of the regenerated plant material needs to be evaluated. OBJECTIVE: In this work, we studied the effect of seed cryopreservation on the phenotypic and molecular characteristics of wild Solanum lycopersicum Mill. plants. METHODS: Thirty-five morphological traits of plants regenerated from cryopreserved seeds were compared to those measured on plants regenerated from non-cryopreserved seeds. RESULT: No statistically significant differences were observed between cryopreserved and non-cryopreserved samples, either in the first or in the second generation post-liquid nitrogen exposure. However, at the molecular level, the genetic analyses performed on the second generation plants germinated from control and cryopreserved seeds using 14 nuclear Simple Sequences Repeats (SSR) markers uncovered some changes in microsatellite length between control and cryopreserved samples. These results confirm at the botanical phenotype level the effectiveness of seed cryostorage for conservation and regeneration of true-to-type S. lycopersicum plants. CONCLUSION: Further experiments are required to clarify potential phenotypic effects of the changes observed in the DNA.
Authors: Ayed M Al-Abdallat; Rida A Shibli; Muhanad W Akash; Manar Rabbaa; Tamara Al-Qudah Journal: Int J Mol Sci Date: 2017-07-21 Impact factor: 5.923