| Literature DB >> 24997786 |
Junhua Li1, Huijue Jia2, Xianghang Cai2, Huanzi Zhong2, Qiang Feng3, Shinichi Sunagawa4, Manimozhiyan Arumugam5, Jens Roat Kultima4, Edi Prifti6, Trine Nielsen7, Agnieszka Sierakowska Juncker8, Chaysavanh Manichanh9, Bing Chen10, Wenwei Zhang10, Florence Levenez6, Juan Wang10, Xun Xu10, Liang Xiao10, Suisha Liang10, Dongya Zhang10, Zhaoxi Zhang10, Weineng Chen10, Hailong Zhao10, Jumana Yousuf Al-Aama11, Sherif Edris12, Huanming Yang13, Jian Wang14, Torben Hansen7, Henrik Bjørn Nielsen8, Søren Brunak8, Karsten Kristiansen15, Francisco Guarner9, Oluf Pedersen7, Joel Doré16, S Dusko Ehrlich17, Peer Bork18, Jun Wang19.
Abstract
Many analyses of the human gut microbiome depend on a catalog of reference genes. Existing catalogs for the human gut microbiome are based on samples from single cohorts or on reference genomes or protein sequences, which limits coverage of global microbiome diversity. Here we combined 249 newly sequenced samples of the Metagenomics of the Human Intestinal Tract (MetaHit) project with 1,018 previously sequenced samples to create a cohort from three continents that is at least threefold larger than cohorts used for previous gene catalogs. From this we established the integrated gene catalog (IGC) comprising 9,879,896 genes. The catalog includes close-to-complete sets of genes for most gut microbes, which are also of considerably higher quality than in previous catalogs. Analyses of a group of samples from Chinese and Danish individuals using the catalog revealed country-specific gut microbial signatures. This expanded catalog should facilitate quantitative characterization of metagenomic, metatranscriptomic and metaproteomic data from the gut microbiome to understand its variation across populations in human health and disease.Entities:
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Year: 2014 PMID： 24997786 DOI： 10.1038/nbt.2942
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908