Suresh Babu Sayana1, Chitra C Khanwelkar2, Venkat Rao Nimmagadda3, Jeevan Mani Babu Dasi4, Vasant R Chavan5, Aruna Kutani6, Karthik Kotagiri7. 1. Ph.D. Scholar, Department of Pharmacology, KIMS University , Karad, Maharashtra, India . 2. Professor & HOD, Department of Pharmacology, KIMS University , Karad, Maharashtra, India . 3. Professor & HOD, Department of Pharmacology, VL College of Pharmacy , Raichur, Karnataka, India . 4. Principal, Vikas College of Pharmacy , Visannapeta, Andhra Pradesh, India . 5. Professor & HOD, Department of Pharmacology, RIMS , Raichur, Karnataka, India . 6. Assistant Professor, Department of Pharmacology, Bojjam Narasimhulu College of Pharmacy , Vinay Nagar, Hyderabad, Andhra Pradesh, India . 7. Drug Safety Associate, Quintiles Technologies India Private Limited , Bengaluru, Karnataka, India .
Abstract
BACKGROUND: In congestive heart failure, nephritis, toxemia of pregnancy, premenstrual tension and hypertension associated with oedema diuretic compounds are much helpful to relieve these conditions. AIMS: To study the diuretic activity of alcoholic extract of roots of Cissampelos pareira by Lipschitz method in albino rats. METHODS AND MATERIAL: Five groups of Albino rats were used to evaluate the diuretic activity of alcoholic extract of roots of Cissampelos pareira by using metabolic cages. The group I serves as normal control received vehicle (2% CMC in normal saline), group II with Furosemide (10 mg/Kg, p.o), Groups III, IV and V with low (100 mg/kg), medium (200 mg/kg), and high (400 mg/kg) doses of alcoholic extract of roots of Cissampelos pareira respectively. Immediately after the alcoholic extract of roots of Cissampelos pareira treatment all the rats were hydrated with saline (15 ml/kg, p.o) and 2 animals placed in each metabolic cage, kept at 21°C±0.5°C. No food and water was made available to animals for 5 hour. The total volume of urine collected with each metabolic cage was measured at the end of 5 hour. Various parameters like total urine volume and concentration of different ions i.e.; Sodium, Potassium , Chloride in the urine were measured. RESULTS: In this model when compared to control group the alcoholic extract of roots of Cissampelos pareira treated groups at different dose levels (100,200 and 400 mg/kg) have noted with significant increase in the urine volume and also significantly enhanced the excretion of Sodium, Potassium and Chloride ions in urine. CONCLUSION: RESULTS showed that single dose administration of standard Furosemide and alcoholic extract of roots of Cissampelos pareira significantly (p<0.05*, p<0.01(**), p<0.001***) increased the urine output along with an increase in elimination of Sodium, Potassium, and Chloride ions. Alcoholic extract of roots of Cissampelos pareira 400 mg/Kg produced a comparable diuretic activity with standard Furosemide.
BACKGROUND: In congestive heart failure, nephritis, toxemia of pregnancy, premenstrual tension and hypertension associated with oedema diuretic compounds are much helpful to relieve these conditions. AIMS: To study the diuretic activity of alcoholic extract of roots of Cissampelos pareira by Lipschitz method in albino rats. METHODS AND MATERIAL: Five groups of Albino rats were used to evaluate the diuretic activity of alcoholic extract of roots of Cissampelos pareira by using metabolic cages. The group I serves as normal control received vehicle (2% CMC in normal saline), group II with Furosemide (10 mg/Kg, p.o), Groups III, IV and V with low (100 mg/kg), medium (200 mg/kg), and high (400 mg/kg) doses of alcoholic extract of roots of Cissampelos pareira respectively. Immediately after the alcoholic extract of roots of Cissampelos pareira treatment all the rats were hydrated with saline (15 ml/kg, p.o) and 2 animals placed in each metabolic cage, kept at 21°C±0.5°C. No food and water was made available to animals for 5 hour. The total volume of urine collected with each metabolic cage was measured at the end of 5 hour. Various parameters like total urine volume and concentration of different ions i.e.; Sodium, Potassium , Chloride in the urine were measured. RESULTS: In this model when compared to control group the alcoholic extract of roots of Cissampelos pareira treated groups at different dose levels (100,200 and 400 mg/kg) have noted with significant increase in the urine volume and also significantly enhanced the excretion of Sodium, Potassium and Chloride ions in urine. CONCLUSION: RESULTS showed that single dose administration of standard Furosemide and alcoholic extract of roots of Cissampelos pareira significantly (p<0.05*, p<0.01(**), p<0.001***) increased the urine output along with an increase in elimination of Sodium, Potassium, and Chloride ions. Alcoholic extract of roots of Cissampelos pareira 400 mg/Kg produced a comparable diuretic activity with standard Furosemide.