Senthamarai S1, Suneel Kumar Reddy A2, Sivasankari S3, Anitha C3, Somasunder V4, Kumudhavathi Ms5, Amshavathani Sk6, Venugopal V7. 1. Associate Professor, Department of Microbiology, Meenakshi Medical College and Research Institute , Enathur, Kachipuram, Tamilnadu, India . 2. 3 Year Post Graduate, Meenakshi Medical College and Research Institute , Enathur, Kachipuram, Tamilnadu, India . 3. Assistant Professor, Department of Microbiology, Meenakshi Medical College and Research Institute , Enathur, Kachipuram, Tamilnadu, India . 4. 2 Year Post Graduate, Meenakshi Medical College and Research Institute , Enathur, Kachipuram, Tamilnadu, India . 5. Tutor, Department of Microbiology, Meenakshi Medical College and Research Institute , Enathur, Kachipuram, Tamilnadu, India . 6. Professor & HOD, Department of Microbiology, Meenakshi Medical College and Research Institute , Enathur, Kachipuram, Tamilnadu, India . 7. Director of PG Studies, Department of Microbiology, Meenakshi Medical College and Research Institute , Enathur, Kachipuram, Tamilnadu, India .
Abstract
PURPOSE: This study was undertaken to analyze the extended spectrum of β lactamase (ESBL), metallo β lactamase (MBL) & AmpC production in Pseudomonas aeruginosa in various clinical samples. MATERIALS & METHODS: One hundred four non repetitive clinical specimens were inoculated onto nutrient agar, blood agar and incubated at 37(o)C overnight. The colonies were tested for oxidase test and other biochemical tests and antibiogram. ESBL screening was done using 3(rd) generation cephalosporins and confirmatory combined double disc test, imipenem-EDTA double disc synergy test for MBL enzyme and AmpC test using Cefoxitin disc. Results & Analysis: Out of 104 P.aeruginosa isolates, 42.30% were ESBL producer, 15.38 % MBL producer and none were AmpC producer. Imipenem, Ofloxacin, and aminoglycosides (amikacin (29.8%) tobramycin (29.8%) and netilmycin (13.46%) has got the better antipseudomonal activity in this study. 43 (41.35%) P.aeruginosa was found to be Multi Drug Resistant (MDR). CONCLUSION: This study highlights the prevalence of ESBL, MBL and MDR P.aeruginosa. Carbapenems and aminoglycosides are promising drugs with antipseudomonal activity in our study.
PURPOSE: This study was undertaken to analyze the extended spectrum of β lactamase (ESBL), metallo β lactamase (MBL) & AmpC production in Pseudomonas aeruginosa in various clinical samples. MATERIALS & METHODS: One hundred four non repetitive clinical specimens were inoculated onto nutrient agar, blood agar and incubated at 37(o)C overnight. The colonies were tested for oxidase test and other biochemical tests and antibiogram. ESBL screening was done using 3(rd) generation cephalosporins and confirmatory combined double disc test, imipenem-EDTA double disc synergy test for MBL enzyme and AmpC test using Cefoxitin disc. Results & Analysis: Out of 104 P.aeruginosa isolates, 42.30% were ESBL producer, 15.38 % MBL producer and none were AmpC producer. Imipenem, Ofloxacin, and aminoglycosides (amikacin (29.8%) tobramycin (29.8%) and netilmycin (13.46%) has got the better antipseudomonal activity in this study. 43 (41.35%) P.aeruginosa was found to be Multi Drug Resistant (MDR). CONCLUSION: This study highlights the prevalence of ESBL, MBL and MDR P.aeruginosa. Carbapenems and aminoglycosides are promising drugs with antipseudomonal activity in our study.
Authors: Siti Nur Atiqah Idris; Mohd Nasir Mohd Desa; Muhammad Nazri Aziz; Niazlin Mohd Taib Journal: Southeast Asian J Trop Med Public Health Date: 2012-01 Impact factor: 0.267