Latha Ragunathan1, G K Poongothai2, Annie Rofeena Sinazer2, Kavitha Kannaiyan3, Hemalatha Gurumurthy3, Nirmala Jaget4, Sethumadhavan Kuthalaramalingam5. 1. Associate Professor, Department of Microbiology, Aarupadai Veedu Medical College and Hospital , Puducherry, India . 2. Post Graduate Student, Department of Microbiology, Aarupadai Veedu Medical College and Hospital , Puducherry, India . 3. Assistant Professor, Department of Microbiology, Aarupadai Veedu Medical College and Hospital , Puducherry, India . 4. Associate Professor, Department of Obstetrics and Gynaecology, Aarupadai Veedu Medical College and Hospital , Puducherry, India . 5. Professor, Department of Microbiology, Aarupadai Veedu Medical College and Hospital , Puducherry, India .
Abstract
BACKGROUND: Vaginal candidiasis is a common gynecological finding among women worldwide. This study was carried out to determine the prevalence of vulvovaginal candidiasis (VVC) along with speciation of Candida with special reference to its antifungal susceptibility pattern to fluconazole and also to evaluate the risk factors responsible for VVC in patients attending our tertiary care hospital in Puducherry, India. MATERIALS AND METHODS: This study was carried out in the tertiary care hospital in Puducherry during the period of August 2010 to September 2012.The study group consisted of 180 women between the age group of 15 to 56 years with the complaints of excessive vaginal discharge, pruritis and pain. Materials used for this study consisted of high vaginal swabs from patients with relevant history, attending Obstetrics & Gynecology department. High vaginal swabs were subjected to direct 10% KOH wet mount microscopy, Gram stain, culture onto Sabouraud's dextrose agar (SDA) & 5% sheep blood agar and susceptibility testing to fluconazole was performed using E-test. RESULTS: Candida was isolated in 40 (22.2 %) women & these consisted of C. albicans 26 (65%), C. glabrata 9 (22.5%), C.tropicalis 3 (7.5%) & C. parapsilosis 2 (5%). Susceptibility test carried out on the 40 isolates revealed that 35 (87.5%) Candida isolates were sensitive to fluconazole, 3 (7.5%) were moderately sensitive and 2 (2.5%) were resistant. Thirty one percent patients had itching as the presenting complaints followed by vaginal discharge (29.4%). CONCLUSION: The high frequency with which C. albicans was recovered in our study and its susceptibility to fluconazole supports the continued use of azole agents for empirical therapy of uncomplicated candidal vulvovaginitis in the community.
BACKGROUND:Vaginal candidiasis is a common gynecological finding among women worldwide. This study was carried out to determine the prevalence of vulvovaginal candidiasis (VVC) along with speciation of Candida with special reference to its antifungal susceptibility pattern to fluconazole and also to evaluate the risk factors responsible for VVC in patients attending our tertiary care hospital in Puducherry, India. MATERIALS AND METHODS: This study was carried out in the tertiary care hospital in Puducherry during the period of August 2010 to September 2012.The study group consisted of 180 women between the age group of 15 to 56 years with the complaints of excessive vaginal discharge, pruritis and pain. Materials used for this study consisted of high vaginal swabs from patients with relevant history, attending Obstetrics & Gynecology department. High vaginal swabs were subjected to direct 10% KOH wet mount microscopy, Gram stain, culture onto Sabouraud's dextrose agar (SDA) & 5% sheep blood agar and susceptibility testing to fluconazole was performed using E-test. RESULTS: Candida was isolated in 40 (22.2 %) women & these consisted of C. albicans 26 (65%), C. glabrata 9 (22.5%), C.tropicalis 3 (7.5%) & C. parapsilosis 2 (5%). Susceptibility test carried out on the 40 isolates revealed that 35 (87.5%) Candida isolates were sensitive to fluconazole, 3 (7.5%) were moderately sensitive and 2 (2.5%) were resistant. Thirty one percent patients had itching as the presenting complaints followed by vaginal discharge (29.4%). CONCLUSION: The high frequency with which C. albicans was recovered in our study and its susceptibility to fluconazole supports the continued use of azole agents for empirical therapy of uncomplicated candidal vulvovaginitis in the community.
Authors: R Goswami; V Dadhwal; S Tejaswi; K Datta; A Paul; R N Haricharan; U Banerjee; N P Kochupillai Journal: J Infect Date: 2000-09 Impact factor: 6.072
Authors: Srujana Mohanty; Immaculata Xess; Fahmi Hasan; Arti Kapil; Suneeta Mittal; Jorge E Tolosa Journal: Indian J Med Res Date: 2007-09 Impact factor: 2.375