Paul B Marshburn1, Allie Giddings2, Stephanie Causby3, Michelle L Matthews3, Rebecca S Usadi3, Nury Steuerwald4, Bradley S Hurst3. 1. Division of Reproductive Endocrinology and Infertility, Carolinas Healthcare System, Charlotte, North Carolina; Department of Obstetrics and Gynecology, Carolinas Healthcare System, Charlotte, North Carolina. Electronic address: paul.marshburn@carolinashealthcare.org. 2. Department of Obstetrics and Gynecology, Carolinas Healthcare System, Charlotte, North Carolina. 3. Division of Reproductive Endocrinology and Infertility, Carolinas Healthcare System, Charlotte, North Carolina; Department of Obstetrics and Gynecology, Carolinas Healthcare System, Charlotte, North Carolina. 4. Cannon Research Center, Carolinas Healthcare System, Charlotte, North Carolina.
Abstract
OBJECTIVE: To determine whether the period of ejaculatory abstinence (EA) influences the total antioxidant capacity (TAC) of semen or lipid peroxidation (LPO) of sperm membranes. DESIGN: A prospective experimental trial. SETTING: Academic medical center for reproductive endocrinology and infertility. PATIENT(S): Forty men from infertile couples planning intrauterine insemination. INTERVENTION(S): Men provided semen specimens after EA periods of 1 and 4 days. MAIN OUTCOME MEASURE(S): Semen analysis, peroxidase staining, and assays for seminal TAC and sperm membrane LPO, with measures compared between days 1 and 4 within individuals (internal control) using paired t tests. RESULT(S): The shorter period of EA (1 day vs. 4 days) resulted in statistically significant decreases in semen volume (-24%), sperm density (-28%), and total sperm count (-3.2%). There was a statistically significant increase in TAC with the shorter period of EA (1 day) compared with 4 days of EA. No difference was detected in sperm membrane LPO comparing 1 day of EA and 4 days of EA. CONCLUSION(S): Higher seminal TAC obtained after a shorter period of EA could diminish oxidative stress-induced sperm damage by a mechanism independent of LPO. Shorter periods of EA may thus improve sperm quality by protecting from reactive oxygen species damage, even though lower numbers of motile sperm are produced after a shorter period of EA. This would be consistent with prior research indicating improved results after intrauterine insemination under these circumstances.
OBJECTIVE: To determine whether the period of ejaculatory abstinence (EA) influences the total antioxidant capacity (TAC) of semen or lipid peroxidation (LPO) of sperm membranes. DESIGN: A prospective experimental trial. SETTING: Academic medical center for reproductive endocrinology and infertility. PATIENT(S): Forty men from infertile couples planning intrauterine insemination. INTERVENTION(S): Men provided semen specimens after EA periods of 1 and 4 days. MAIN OUTCOME MEASURE(S): Semen analysis, peroxidase staining, and assays for seminal TAC and sperm membrane LPO, with measures compared between days 1 and 4 within individuals (internal control) using paired t tests. RESULT(S): The shorter period of EA (1 day vs. 4 days) resulted in statistically significant decreases in semen volume (-24%), sperm density (-28%), and total sperm count (-3.2%). There was a statistically significant increase in TAC with the shorter period of EA (1 day) compared with 4 days of EA. No difference was detected in sperm membrane LPO comparing 1 day of EA and 4 days of EA. CONCLUSION(S): Higher seminal TAC obtained after a shorter period of EA could diminish oxidative stress-induced sperm damage by a mechanism independent of LPO. Shorter periods of EA may thus improve sperm quality by protecting from reactive oxygen species damage, even though lower numbers of motile sperm are produced after a shorter period of EA. This would be consistent with prior research indicating improved results after intrauterine insemination under these circumstances.
Authors: Barbara Ferreira da Silva; Chen Meng; Dominic Helm; Fiona Pachl; Jürgen Schiller; Emad Ibrahim; Charles M Lynne; Nancy L Brackett; Ricardo Pimenta Bertolla; Bernhard Kuster Journal: Mol Cell Proteomics Date: 2016-01-26 Impact factor: 5.911
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