| Literature DB >> 24992671 |
Giovanni Faggioni1, Riccardo De Santis2, Alice Pomponi3, Massimo Fantini4, Giovanni Savini5, Federica Monaco6, Andrea Polci7, Roberto Bei8, Florigio Lista9.
Abstract
Following its spread in the USA, West Nile Virus (WNV) has reemerged in the Mediterranean basin with a renewed pathogenicity. The introduction of WNV lineage 2 in Europe and its co-circulation with lineage 1 has resulted in a continuously changing epidemiological scenario, highlighting the importance of differential detection of the two lineages. The paper describes a new real-time PCR method for the detection and genotyping of the two main lineages of WNV. The method requires a single pair of primers and probes and is based on the analysis of highly conserved consensus sequences detected in the 5' terminus of the viral genome.Entities:
Keywords: Flavivirus; Melting curve analysis; Molecular detection; Virus genotyping; WNV
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Year: 2014 PMID： 24992671 DOI： 10.1016/j.jviromet.2014.06.020
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014