Literature DB >> 24989793

A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications.

K Engelmark Cassimjee1, M Kadow, Y Wikmark, M Svedendahl Humble, M L Rothstein, D M Rothstein, J-E Bäckvall.   

Abstract

A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG™, is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.

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Year:  2014        PMID： 24989793     DOI： 10.1039/c4cc02605e

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  13 in total

1.  Thiol Specific and Tracelessly Removable Bioconjugation via Michael Addition to 5-Methylene Pyrrolones.

Authors:  Yingqian Zhang; Xiaoping Zhou; Yonghui Xie; Marc M Greenberg; Zhen Xi; Chuanzheng Zhou
Journal:  J Am Chem Soc       Date:  2017-04-20       Impact factor: 15.419

2.  Improvement of the Process Stability of Arylmalonate Decarboxylase by Immobilization for Biocatalytic Profen Synthesis.

Authors:  Miriam Aßmann; Carolin Mügge; Sarah Katharina Gaßmeyer; Junichi Enoki; Lutz Hilterhaus; Robert Kourist; Andreas Liese; Selin Kara
Journal:  Front Microbiol       Date:  2017-03-16       Impact factor: 5.640

3.  Smart Nanoparticles for Selective Immobilization of Acid Phosphatases.

Authors:  Flóra Nagy; Gábor Tasnádi; Diána Balogh-Weiser; Evelin Bell; Mélanie Hall; Kurt Faber; László Poppe
Journal:  ChemCatChem       Date:  2018-07-17       Impact factor: 5.686

4.  Streamlined Preparation of Immobilized Candida antarctica Lipase B.

Authors:  Karim Engelmark Cassimjee; Peter Hendil-Forssell; Alexey Volkov; Anne Krog; Jostein Malmo; Trond Erik V Aune; Wolfgang Knecht; Iain R Miskelly; Thomas S Moody; Maria Svedendahl Humble
Journal:  ACS Omega       Date:  2017-12-06

5.  B-factor Guided Proline Substitutions in Chromobacterium violaceum Amine Transaminase: Evaluation of the Proline Rule as a Method for Enzyme Stabilization.

Authors:  Henrik Land; Jonatan C Campillo-Brocal; Maria Svedendahl Humble; Per Berglund
Journal:  Chembiochem       Date:  2019-03-19       Impact factor: 3.164

6.  Highly efficient production of chiral amines in batch and continuous flow by immobilized ω-transaminases on controlled porosity glass metal-ion affinity carrier.

Authors:  Wesley Böhmer; Tanja Knaus; Alexey Volkov; Thierry K Slot; N Raveendran Shiju; Karim Engelmark Cassimjee; Francesco G Mutti
Journal:  J Biotechnol       Date:  2018-12-11       Impact factor: 3.307

Review 7.  Recent Trends in Enzyme Immobilization-Concepts for Expanding the Biocatalysis Toolbox.

Authors:  Hans-Jürgen Federsel; Thomas S Moody; Steve J C Taylor
Journal:  Molecules       Date:  2021-05-10       Impact factor: 4.411

8.  Hydrogen-Borrowing Alcohol Bioamination with Coimmobilized Dehydrogenases.

Authors:  Wesley Böhmer; Tanja Knaus; Francesco G Mutti
Journal:  ChemCatChem       Date:  2018-01-11       Impact factor: 5.686

9.  Structural Changes of Hierarchically Nanoporous Organosilica/Silica Hybrid Materials by Pseudomorphic Transformation.

Authors:  Malina Bilo; Maximilian Münzner; Christian Küster; Dirk Enke; Young Joo Lee; Michael Fröba
Journal:  Chemistry       Date:  2020-06-11       Impact factor: 5.236

10.  Parallel Interconnected Kinetic Asymmetric Transformation (PIKAT) with an Immobilized ω-Transaminase in Neat Organic Solvent.

Authors:  Wesley Böhmer; Lucien Koenekoop; Timothée Simon; Francesco G Mutti
Journal:  Molecules       Date:  2020-05-03       Impact factor: 4.411
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