Literature DB >> 2498492

Evaluation of a competitive antibody enzyme immunoassay for specific diagnosis of Chagas' disease.

W R Cuna1, C Rodriguez, F Torrico, D Afchain, M Loyens, P Desjeux.   

Abstract

A competitive enzyme immunoassay based on the use of a monoclonal antibody (MAb) specific for "component 5" of Trypanosoma cruzi was evaluated. The antigenicity and immunogenicity of this component has been observed in natural and experimental infections. The studies were conducted in an area of Bolivia where mixed infections with Leishmania braziliensis are frequent and present a problem in the accurate diagnosis of T. cruzi infections. The specificity and sensitivity of this assay as compared to the indirect immunofluorescence and ELISA tests were demonstrated. The present test has proved to be more specific than the immunofluorescence and ELISA tests.

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Year:  1989        PMID： 2498492

Source DB:  PubMed          Journal:  J Parasitol        ISSN： 0022-3395            Impact factor:   1.276


  4 in total

1.  Development and comparison of enzyme immunoassays for diagnosis of Chagas' disease using fixed forms of Trypanosoma cruzi (Epimastigotes, Amastigotes, and Trypomastigotes) and assessment of antigen stability for the three assays.

Authors:  Mariolga Berrizbietia; Momar Ndao; Marcelo Gottschalk; Alberto Aché; Fabio Vásquez; Sonia Lacouture; Mehudy Medina; Brian J Ward
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

2.  Mass screening for Trypanosoma cruzi infections using the immunofluorescence, ELISA and haemagglutination tests on serum samples and on blood eluates from filter-paper.

Authors:  F Zicker; P G Smith; A O Luquetti; O S Oliveira
Journal:  Bull World Health Organ       Date:  1990       Impact factor: 9.408

3.  Design, construction, and evaluation of a specific chimeric antigen to diagnose chagasic infection.

Authors:  Sebastián Aguirre; Ariel M Silber; Maria Edileuza F Brito; María E Ribone; Claudia M Lagier; Iván S Marcipar
Journal:  J Clin Microbiol       Date:  2006-10       Impact factor: 5.948

4.  Development and Application of an ELISA Assay Using Excretion/Secretion Proteins from Epimastigote Forms of T. cruzi (ESEA Antigens) for the Diagnosis of Chagas Disease.

Authors:  Mariolga Berrizbeitia; Milagros Figueroa; Brian J Ward; Jessicca Rodríguez; Alicia Jorquera; Maria A Figuera; Leomerys Romero; Momar Ndao
Journal:  J Trop Med       Date:  2012-09-25
  4 in total

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