B Spoto1, E Di Betta2, F Mattace-Raso3, E Sijbrands3, A Vilardi2, R M Parlongo1, P Pizzini1, A Pisano1, W Vermi4, A Testa1, S Cutrupi1, G D'Arrigo1, S Lonardi4, G Tripepi1, G Cancarini5, C Zoccali6. 1. CNR-IFC, Institute of Clinical Physiology, Clinical Epidemiology and Physiopathology of Renal Diseases and Hypertension, Reggio Calabria, Italy. 2. Division of General Surgery 1, A.O. Spedali Civili di Brescia, Italy. 3. Department of Internal Medicine, Erasmus University Medical Centre, Rotterdam, The Netherlands. 4. Section of Pathology, University of Brescia, Italy. 5. Unit of Nephrology, A.O. Spedali Civili and University, Brescia, Italy. 6. CNR-IFC, Institute of Clinical Physiology, Clinical Epidemiology and Physiopathology of Renal Diseases and Hypertension, Reggio Calabria, Italy. Electronic address: carmine.zoccali@tin.it.
Abstract
BACKGROUND AND AIMS: Pro-inflammatory molecules produced by adipose tissue have been implicated in the risk of cardiovascular (CV) disease in obesity. We investigated the expression profile of 19 pro-inflammatory and seven anti-inflammatory genes in subcutaneous adipose tissue (SAT) and in visceral adipose tissue (VAT) in 44 severely obese individuals who underwent bariatric surgery. METHODS AND RESULTS: SAT and VAT expressed an identical series of pro-inflammatory genes. Among these genes, 12 were significantly more expressed in SAT than in VAT while just one (IL18) was more expressed in VAT. The remaining genes were equally expressed. Among pro-inflammatory cytokines, both IL6 and IL8 were about 20 times more intensively expressed in SAT than in VAT. The expression of nine genes was highly associated in SAT and VAT. Only for three pro-inflammatory cytokines (IL8, IL18, SAA1) in SAT the gene expression in adipose tissue associated with the circulating levels of the corresponding gene products while no such an association was found as for VAT. CONCLUSIONS: The expression of critical pro-inflammatory genes is substantially higher in SAT than in VAT in individuals with morbid obesity. The variability in circulating levels of pro-inflammatory cytokines is, in small part and just for three pro-inflammatory cytokines, explained by underlying gene expression in SAT but not in VAT. These results point to a compartment-specific adipose tissue contribution to inflammation in obesity and indicate that abdominal SAT contributes more than VAT to the pro-inflammatory milieu associated with severe obesity.
BACKGROUND AND AIMS: Pro-inflammatory molecules produced by adipose tissue have been implicated in the risk of cardiovascular (CV) disease in obesity. We investigated the expression profile of 19 pro-inflammatory and seven anti-inflammatory genes in subcutaneous adipose tissue (SAT) and in visceral adipose tissue (VAT) in 44 severely obese individuals who underwent bariatric surgery. METHODS AND RESULTS: SAT and VAT expressed an identical series of pro-inflammatory genes. Among these genes, 12 were significantly more expressed in SAT than in VAT while just one (IL18) was more expressed in VAT. The remaining genes were equally expressed. Among pro-inflammatory cytokines, both IL6 and IL8 were about 20 times more intensively expressed in SAT than in VAT. The expression of nine genes was highly associated in SAT and VAT. Only for three pro-inflammatory cytokines (IL8, IL18, SAA1) in SAT the gene expression in adipose tissue associated with the circulating levels of the corresponding gene products while no such an association was found as for VAT. CONCLUSIONS: The expression of critical pro-inflammatory genes is substantially higher in SAT than in VAT in individuals with morbid obesity. The variability in circulating levels of pro-inflammatory cytokines is, in small part and just for three pro-inflammatory cytokines, explained by underlying gene expression in SAT but not in VAT. These results point to a compartment-specific adipose tissue contribution to inflammation in obesity and indicate that abdominal SAT contributes more than VAT to the pro-inflammatory milieu associated with severe obesity.
Authors: P Mauriège; D R Joanisse; S CasparBauguil; A Cartier; I Lemieux; J Bergeron; S Biron; P Marceau; D Richard Journal: J Physiol Biochem Date: 2015-10-10 Impact factor: 4.158
Authors: Marta Izabela Jonas; Alina Kurylowicz; Zbigniew Bartoszewicz; Wojciech Lisik; Maurycy Jonas; Zbigniew Wierzbicki; Andrzej Chmura; Piotr Pruszczyk; Monika Puzianowska-Kuznicka Journal: Int J Mol Sci Date: 2015-10-28 Impact factor: 5.923