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Literature DB >> 2498282

Proteins that interact with GTP during sporulation of Bacillus subtilis.

Abstract

During sporulation of Bacillus subtilis, several proteins were shown to interact with GTP in specific ways. UV light was used to cross-link [alpha-32P]GTP to proteins in cell extracts at different stages of growth. After electrophoresis, 11 bands of radioactivity were found in vegetative cells, 4 more appeared during sporulation, and only 9 remained in mature spores. Based on the labeling pattern with or without UV light to cross-link either [alpha-32P]GTP or [gamma-32P]GTP, 11 bands of radioactivity were apparent guanine nucleotide-binding proteins, and 5 bands appeared to be phosphorylated and/or guanylated. Similar results were found with Bacillus megaterium. Assuming that GTP might be a type of signal for sporulation, it could interact with and regulate proteins by at least three mechanisms.

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Year: 1989 PMID： 2498282 PMCID： PMC209994 DOI： 10.1128/jb.171.6.2915-2918.1989

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

18 in total

Proteins that interact with GTP during sporulation of Bacillus subtilis.

1. A GTP-binding protein of Escherichia coli has homology to yeast RAS proteins.

Review 2. G proteins: transducers of receptor-generated signals.

Review 3. Protein phosphorylation in prokaryotes.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Odorant-sensitive adenylate cyclase may mediate olfactory reception.

6. An affinity labeling of ras p21 protein and its use in the identification of ras p21 in cellular and tissue extracts.

7. Biochemical studies on glucose initiated germination in Bacillus megaterium.

8. Protein phosphorylation in Escherichia coli and purification of a protein kinase.

9. Measurement of protein using bicinchoninic acid.

10. Germination of Bacillus megaterium spores after various extraction procedures.

1. YsxC, a putative GTP-binding protein essential for growth of Bacillus subtilis 168.

2. Identification of proteins phosphorylated by ATP during sporulation of Bacillus subtilis.

3. A structured model for vegetative growth and sporulation in Bacillus thuringiensis.

4. The SpoIIAA protein of Bacillus subtilis has GTP-binding properties.

5. Specific in vitro guanylylation of a 43-kilodalton membrane-associated protein of Streptomyces coelicolor.