Caco-2 replace or refine?

Multiple screening techniques have been developed to gain simplicity and rapidness in prediction of human intestinal permeability. The most extensively used method for years has been the Caco-2 cell monolayers. Are the less time- and resource-consuming artificial membranes and computational-based predictions on their way to replace the Caco-2 cells? The importance of mechanistic approaches and correct interpretation of the data using the techniques available is compared and discussed in this article.: