| Literature DB >> 24980795 |
Ekaterina Shimanovskaya1, Valeria Viscardi2, Johannes Lesigang1, Molly M Lettman2, Renping Qiao1, Dmitri I Svergun3, Adam Round4, Karen Oegema5, Gang Dong6.
Abstract
Plk4 family kinases control centriole assembly. Plk4s target mother centrioles through an interaction between their cryptic polo box (CPB) and acidic regions in the centriolar receptors SPD-2/Cep192 and/or Asterless/Cep152. Here, we report a crystal structure for the CPB of C. elegans ZYG-1, which forms a Z-shaped dimer containing an intermolecular β sheet with an extended basic surface patch. Biochemical and in vivo analysis revealed that electrostatic interactions dock the ZYG-1 CPB basic patch onto the SPD-2-derived acidic region to promote ZYG-1 targeting and new centriole assembly. Analysis of a different crystal form of the Drosophila Plk4 (DmPlk4) CPB suggests that it also forms a Z-shaped dimer. Comparison of the ZYG-1 and DmPlk4 CPBs revealed structural changes in the ZYG-1 CPB that confer selectivity for binding SPD-2 over Asterless-derived acidic regions. Overall, our findings suggest a conserved mechanism for centriolar docking of Plk4 homologs that initiate daughter centriole assembly.Entities:
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Year: 2014 PMID： 24980795 PMCID： PMC4126857 DOI： 10.1016/j.str.2014.05.009
Source DB: PubMed Journal: Structure ISSN： 0969-2126 Impact factor: 5.006