Yavuz Simşek1, Hakan Parlakpınar2, Uğur Turhan3, Mehmet Emin Tağluk4, Burhan Ateş5. 1. Department of Obstetrics and Gynecology, Kırıkkale University Faculty of Medicine, Kırıkkale, Turkey. 2. Department of Medical Pharmacology, İnönü University Faculty of Medicine, Malatya, Turkey. 3. Department of Obstetrics and Gynecology, İnönü University Faculty of Medicine, Malatya, Turkey. 4. Department of Electrical and Electronics, İnönü University Faculty of Engineering, Malatya, Turkey. 5. Department of Chemistry, İnönü University Faculty of Science, Malatya, Turkey.
Abstract
OBJECTIVE: In this experimental study, we aimed to investigate the role of melatonin on uterine myoelectrical activity of non-pregnant rats. MATERIAL AND METHODS: Forty-six female rats were assigned to six groups: (1) control; (0.2 mL 0.9% NaCl was injected intravenously (IV), n=6); (2) melatonin applied as 0.4 mg/kg/IV (n=8); (3) melatonin applied as 4 mg/kg/IV (n=8); (4) single dose of oxytocin (100 mU/kg) injected IV (n=8); (5) melatonin (0.4 mg/kg) plus oxytocin (100 mU/kg) (n=8); and (6) melatonin (4 mg/kg) plus oxytocin (100 mU/kg) injected IV (n=8). Each rat underwent a laparotomy, and uterine myoelectrical signals were recorded. The mean spectrum, averaged over the spectral content of signals in each group, was compared. RESULTS: Melatonin induced uterine myoelectrical activity in a dose-dependent manner. Treatment of melatonin after oxytocin suppressed the mean power of the signals. Serum melatonin concentrations were significantly higher in melatonin-treated rats. CONCLUSION: Melatonin itself at two different dose levels was found to be equally effective in stimulating the uterine electrical signals, although oxytocin-induced uterine electrical activity was suppressed by melatonin. These findings merit further investigations on the possible beneficial role of melatonin in the treatment of conditions associated with abnormal uterine activity.
OBJECTIVE: In this experimental study, we aimed to investigate the role of melatonin on uterine myoelectrical activity of non-pregnant rats. MATERIAL AND METHODS: Forty-six female rats were assigned to six groups: (1) control; (0.2 mL 0.9% NaCl was injected intravenously (IV), n=6); (2) melatonin applied as 0.4 mg/kg/IV (n=8); (3) melatonin applied as 4 mg/kg/IV (n=8); (4) single dose of oxytocin (100 mU/kg) injected IV (n=8); (5) melatonin (0.4 mg/kg) plus oxytocin (100 mU/kg) (n=8); and (6) melatonin (4 mg/kg) plus oxytocin (100 mU/kg) injected IV (n=8). Each rat underwent a laparotomy, and uterine myoelectrical signals were recorded. The mean spectrum, averaged over the spectral content of signals in each group, was compared. RESULTS:Melatonin induced uterine myoelectrical activity in a dose-dependent manner. Treatment of melatonin after oxytocin suppressed the mean power of the signals. Serum melatonin concentrations were significantly higher in melatonin-treated rats. CONCLUSION:Melatonin itself at two different dose levels was found to be equally effective in stimulating the uterine electrical signals, although oxytocin-induced uterine electrical activity was suppressed by melatonin. These findings merit further investigations on the possible beneficial role of melatonin in the treatment of conditions associated with abnormal uterine activity.
Authors: Russel J Reiter; Ana Coto-Montes; Jose Antonio Boga; Lorena Fuentes-Broto; Sergio Rosales-Corral; Duan-Xian Tan Journal: Neuro Endocrinol Lett Date: 2011 Impact factor: 0.765
Authors: Frank Steffens; Xiao-Bo Zhou; Ulrike Sausbier; Claudia Sailer; Karin Motejlek; Peter Ruth; James Olcese; Michael Korth; Thomas Wieland Journal: Mol Endocrinol Date: 2003-07-17